经实时荧光定量逆转录聚合酶链反应验证了基因芯片准确可靠。
The results of realtime RTPCR proved the accuracy of the gene chip.
方法应用荧光定量逆转录-多聚酶链反应（RT -PCR）检测了30例急性白血病患者和8例正常人外周血MDR1基因的表达。
Methods MDR1 gene expression in case of 30 leukemia and 8 healthy persons' peripheral blood have been detested by fluorescence-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
采用半定量逆转录多聚酶链反应(RT - PCR)检测局部内皮素系统ET - 1、ETAR、ETBR及ECE的基因表达。
The gene expression of ET-1, ETAR, ETBR and ECE was evaluated by semi-quantitative reverse transcription polymerase chain response (RT-PCR).
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
方法采用半定量pcr方法检测了我国四种小型猪内源性逆转录病毒基因的拷贝数。
Methods Semi-quantitative PCR method was used in this research to estimate PERV copy-number of four mini-pigs in China.
应用逆转录聚合酶链反应(RT - PCR)半定量分析大鼠脑放射性损伤后海马区在不同时间、不同剂量水平IL - 6基因转录的动态表达。
Semiquantitative analysis of IL-6 in the hippocampus was done at different time and dose level after whole-brain irradiation with reverse-transcription polymerase chain reaction (RT-PCR).
应用逆转录PCR结合同位素定量分析，对32例儿童白血病患者的多源耐药基因表达水平进行了研究。
Quantitative RT PCR method were used to detect the mRNA levels of multidrug resistance gene in 32 leukemia children.
应用逆转录PCR结合同位素定量分析，对32例儿童白血病患者的多源耐药基因表达水平进行了研究。
Quantitative RT PCR method were used to detect the mRNA levels of multidrug resistance gene in 32 leukemia children.
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