目的建立变形菌随机扩增dna多态性分型技术,应用于临床菌株流行病学调查。
OBJECTIVE To establish the random amplification of polymorphic DNA (RAPD) typing technology for Proteus strains and apply it in the epidemiology investigation.
方法建立随机扩增多态性DNA聚丙酰胺凝胶电泳(RAPD-PAGE)指纹分型法;
Methods To establish a fingerprinting method by random amplified polymorphic DNA and polyacrylamide gel electrophoresis (RAPD-PAGE).
目的研究中国汉族人群abo基因多态性,并将基因分型技术用于解决临床输注中血型血清学难题。
Objective to study ABO gene polymorphism of Chinese Han population and apply ABO genotyping technology to solve the serological problems of clinical transfusion.
采用脉冲场凝胶电泳(PFGE)和随机引物多态性DNA(RAPD)分型对128株CRAB进行分子流行病学研究;
The homology of 128 isolates of CRAB was determined by both pulsed field gel electrophoresis (PFGE) and randomly amplified polymorphism DNA (RAPD).
方法:综述近几年具遗传多态性代谢酶的探针药物、代谢分型方法以及影响因素。
METHOD:The probe drugs of drug metabolism, methods of phenotype and related factors were reviewed.
目的应用随机扩增DNA多态性(RAPD)技术对医院感染的粘质沙雷菌进行基因分型。
Objective To evaluate the genetic polymorphism of Serratia marcescens by randomly amplified polymorphic DNA (RAPD) method.
方法在140名汉族健康人的外周血中,应用聚合酶链反应(PCR)限制性片段长度多态性分析(RFLP)及多重PCR技术,进行NAT1等位基因分型研究。
Methods Using multiple PCR and PCR-RFLP methods, we studied the NAT1 genotypes and its genetic polymorphisms of the peripheral blood samples from 140 Han people.
目的建立随机扩增多态性DNA技术(RAPD)对甲氧西林耐药溶血性葡萄球菌(MRSH)的分型方法。
Objective To establish the Random Amplified Polymorphic DNA (RAPD) Technique for typing methicillin resistant Staphylococcus haemolyticus (MRSH).
目的探讨雄激素受体基因CAG多态性与痤疮中医分型的相关性。
Objective to study the relativity of the CAG polymorphisms of androgen receptor gene of the patients with acne and syndrome differentiation and typing of TCM.
SNP位点是目前基因多态性研究的主要内容,包括检测分型和功能活性研究两个层次,已经建立了高度自动化和高通量的SNP检测分型技术。
Single Nucleotide polymorphism (SNP) site is the main content of genetic polymorphism study, which includes detection of gene type and research on function and activity.
方法在小样本中测序检测TLR2基因中可能存在的基因多态性,再用连接酶特异检测技术在大样本进行SNP分型,并通过统计学方法分析基因多态性与结核病易感性之间相关性。
Methods TLR2 gene polymorphisms were analyzed by DNA sequencing in a small amount of samples. SNPs in TLR2 gene were detected by ligase detection technique in a large amount of samples.
方法:选择符合入选标准的高血压患者336例,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法,进行ACE2基因分型。
Methods:336 hypertension patients were recruited in this trail. The distribution of ACE2 gene A9570G polymorphism was analyzed by PCR-RFLP in all participants.
方法:选择符合入选标准的高血压患者336例,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法,进行ACE2基因分型。
Methods:336 hypertension patients were recruited in this trail. The distribution of ACE2 gene A9570G polymorphism was analyzed by PCR-RFLP in all participants.
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