整个实验表明通过注射GRF基因质粒可以提高犊牛的生产性能。
The whole trials results showed injection of GRF gene plasmid could improve growth performance of calves.
通过转化绿色荧光蛋白基因质粒，对阿特拉津降解基因工程菌进行标记。
The atrazine-degrading genetically engineered microorganism(GEM) was labeled by transforming a plasmid containing green fluorescent protein(GFP) gene.
我们使用了依赖HCVIRES表达萤火虫荧光素酶的报告基因质粒。
We used a reporter gene plasmid in which firefly luciferase expression is dependent on the HCV IRES.
试验结果表明：导入不同剂量GRF基因质粒，整个饲养试验期中各处理组的生产性能都好于对照组。
The results showed that the growth performance of every treatments injecting different dosage of GRF gene plasmid were better than control to some extend in whole trials.
Church回忆第一次见到了质粒和基因序列的情形-青霉素酶和胰岛素，阻遏物和干扰素。
Church recalls seeing plasmid and gene sequences for the first time-penicillinase and insulin, the lac repressor and interferon.
标本中的NDM-1基因多由质粒携带，来自英国和钦奈者极易转移而来自哈利亚纳的NDM-1却不容易被结合。
Most isolates carried the NDM-1 gene on plasmids: those from UK and Chennai were readily transferable whereas those from Haryana were not conjugative.
其中一个为细菌加入新基因的方法是引入质粒。
One of the ways to introduce genes into a bacterium is to introduce a plasmid into it.
结果：散发性霍乱以无毒株为主，质粒及肠毒素基因的携带率极低，但耐药性有所增加。
Results: The sporadic outbreak of vibrio cholera has some characters: including non toxic strain infection, low detection rate of plasmid and ct gene and increase of drug resistance.
实验结果表明，以TK基因构建的重组载体质粒可用于外源基因的重组表达研究。
The result showed that the transfer vector plasmid constructed with FPVTK genes can be used to express foreign gene in FPV recombinant.
建立了筛选质粒表达文库和克隆免疫原基因的技术方法。
We established the techniques of screening the plasmid expression library and cloning the immunogen genes.
应用花生胚小叶再生体系，以农杆菌介导法对质粒上的基因转化进行初步探讨。
Applying to leaflet regeneration system of peanut, genetic transformation of peanut using Agrobacterium-mediated approach were carrying on the research.
目的从人外周血淋巴细胞中克隆出cd 81基因，构建真核表达质粒，并在COS - 7细胞中进行表达。
Aim to clone human CD81 gene from peripheral blood lymphocytes, and construct its eukaryotic expression vector, and then express it in COS-7 cells.
目的研究电脉冲介导的质粒红细胞生成素(EPO)基因肌肉转移效率以及对肾性贫血的治疗作用。
Objective to investigate the effects of erythropoietin (EPO) gene transfer into skeleton muscle mediated by electroporation on renal anemia.
方法：将基因内翻译起始序列合成后克隆到上游含有起始密码和无起始密码的报告质粒中，研究表达水平的差异。
Methods:One of the three internal translation initiation region(TIR) was chemically synthesized and cloned into the report plasmid, and the expression of the report gene was studied.
目的：构建弓形虫棒状体蛋白（ROP2 ）基因重组质粒并在大肠杆菌中表达，用于筛选弓形虫新的诊断抗原和疫苗分子。
Objective:To construct a recombinant plasmid containing rhoptry protein 2(ROP2)gene of Toxoplasma gondii and express it in E. coli for selection of diagnostic antigen and vaccine candidate.
目的：克隆大鼠神经营养因子4全长基因，构建真核细胞表达质粒。
AIM: to clone rat neurotrophin-4 (NT-4) total gene and construct expression plasmid for prokaryotic cells.
目的构建柯萨奇病毒（CV）B1/B3型二价VP1基因免疫质粒，并探讨其免疫原性。
Objective To construct a bivalent VP1 gene vaccine against Coxsackie virus B1 and B3 (CVB1 and CVB3) and to test its immunogenicity in mice.
为构建其启动外源基因的质粒表达载体作准备。
It was therefore preparation for constructing an expression plasmid vector to express exogenous genes.
构建小鼠il -4截短型基因真核表达质粒，表达小鼠il - 4受体拮抗体蛋白。
To clone mouse interleukin 4 (mIL-4) truncated gene, construct its eukaryotic expression plasmid pFB-mIL4 and express the truncated protein (murine IL-4 receptor antagonist).
质粒接合实验定位耐药基因；
Drug resistant gene were positioned by plasmids conjugation experiments.
结论：高免疫原性的抑制素表达质粒的构建为利用抑制素基因免疫技术诱导单胎动物生多胎奠定了基础。
Conclusion: the fusion gene expression vector was successfully constructed, and it set up the basis of inhibin gene immunization to induce multiple bear for single birth animals.
方法：采用血清学和分子生物学相结合的方法，研究深圳地区散发不同血清型霍乱弧菌的生化特性、质粒、肠毒素基因携带和耐药状况。
Methods: With serological and molecular techniques, we study chemical property, plasmid, CT gene and drug resistance of sporadic outbreak of vibro cholera in Shenzhen city.
目的构建以融合蛋白形式在大肠杆菌中高效表达心钠素的重组质粒，稳定高效地获得基因工程产品心钠素。
Objective To construct recombinant plasmid with human atrial natriuretic peptide (ANP) gene in fusion form for stable and high level expression of genetic engineering product ANP in E. coli system.
构建含蛋白转导结构域(PTD)与脑源性神经营养因子(BDNF)融合基因的质粒，并在大肠肝菌中表达。
To construct a recombinant plasmid containing protein transduction domain (PTD) and brain derived neurotrophic factor (BDNF) fusion gene and express in e.
目的构建弓形虫棒状体蛋白2 （ROP2 ）基因重组质粒。
Objective To construct a recombinant plasmid containing rhoptry protein 2 (ROP2) gene of Toxoplasma gondii .
将文库菌落印迹至尼龙膜，分区培养提取质粒DNA ，建立基因池，并分别转染NIH/3T3细胞。
The plasmids in cDNA library and in gene pools were extracted and NIH/3T3 cells were transfected respectively.
目的：探讨在不锈钢冠状动脉支架上携带质粒基因，为心血管再狭窄基因治疗的临床应用提供试验依据。
Objective: to investigate stainless steel coronary stent-based plasmid gene delivery system and evaluate its feasibility and effectiveness to gene therapy of cardiovascular restenosis.
结果表明：同一质粒可编码一个至数个耐药性基因，不同质粒可以携带相同的耐药性基因，并且这些质粒可以传递多种耐药性。
The result showed that a plasmid can encode single or several resistance genes, and different plasmid also can have same resistance genes. These plasmids can transform multiple resistance.
目的比较脂质体介导法和裸质粒直接转染法导入CYP2J3基因表达的效率。
Objective To study the expression efficiency of CYP2J3 gene in rat smooth muscle cells (SMCs) by liposome mediated and naked plasmid transfection.
W1菌体质粒和染色体提取实验表明，其降解基因位于染色体上。
Plasmid and chromosome extraction experiments indicate that the degradation genes of W1 are on chromosome.
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