方法采用中国药典2005年版一部规定的常规法、培养基稀释法进行菌回收率比较试验。
Methods Common methods in Chinese Pharmacopoeia(edition 2005) were used to cultivate culture media dilution, using recovery rate of the bacteria in the comparison experiment.
经回收率试验确定苯酚-硫酸法为检测此EPS的方法。
The method of detection of EPS was determined as phenol-sulfuric acid method by the experiment of recovery rate.
当试验料为苜蓿、稻谷和玉米-豆粕-苜蓿型配合料时，AIA含量比较高，回收率分别为98.20%，102.36%，91.65%，较为理想，可以使用AIA指示剂法。
The AIA content in groups with alfalfa, paddy and corn-soybean-alfalfa was high, and the recovery was 98.20%, 102.36% and 91.65% respectively, and therefore, the AIA method can be used.
结果：采用培养基稀释法，各试验菌在样品中的回收率均达到70%以上。
Results: In group of medium, the recoveries of the 5 positive control trains in the analysis were all over 70%.
采用培养基稀释法，所有验证菌试验组回收率均大于70%。
While, the recoveries in all tests were above 70% by culture medium-diluting method.
采用培养基稀释法，所有验证菌试验组回收率均大于70%。
While, the recoveries in all tests were above 70% by culture medium-diluting method.
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