目的探讨反义vegf基因和内皮抑素基因联合转染在抑制肿瘤血管生成和肿瘤生长转移中的作用。
Objective to explore the co operative inhibitory effect of antisense VEGF gene and endostatin gene transfection on tumor angiogenesis, tumor growth and metastasis of lung cancer.
方法应用酶联免疫吸附试验(ELISA法)，测定44例原发性肺癌、20例肺部良性疾病和20例正常人血清内皮抑素含量。
Methods Serum levels of endostatin were measured by an enzyme-linked immunosorbent assay (ELISA) in 44 patients with lung cancer, 20 patients with benign lung diseases and 20 normal persons.
方法用PCR方法扩增il_3信号肽序列和人内皮抑素基因，然后采用重叠延伸pcr拼接的方法将两者拼接和扩增。
METHODS IL_3 signal sequence and human endostatin gene were amplified with PCR and spliced with overlap extension PCR.
应用免疫组织化学染色方法，观察各组心肌梗死大鼠缺血心肌中内皮抑素、VEGF的表达和新生血管密度。
The sham group was normal control group (eight rats in each group). The expression of endostatin, VEGF and MVD in ischemic myocardium were observed by immunohistochemistry.
目的：观察心肌梗死后大鼠不同时间缺血心肌中内皮抑素的表达及其与新生血管密度和血管内皮生长因子(VEGF)的关系。
AIM: To study the expression of endostatin in ischemic myocardium of myocardial infarction (mi) rats in various periods and the correlation with VEGF expression and microvascular density (MVD).
目的：研究洛铂联合重组人血管内皮抑素（恩度）治疗肺癌恶性胸腔积液的有效性和安全性。
Objective: To study the efficacy and safety of intrapleural injection of lobaplatin combined with endostar in treatment of lung cancer with malignant pleural effusion.
目的：研究洛铂联合重组人血管内皮抑素（恩度）治疗肺癌恶性胸腔积液的有效性和安全性。
Objective: To study the efficacy and safety of intrapleural injection of lobaplatin combined with endostar in treatment of lung cancer with malignant pleural effusion.
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