目的:克隆长双歧杆菌ncc 2705株果糖结合蛋白bl 0033的基因,利用大肠杆菌表达GST -BL 0033融合蛋白并纯化。
Objective: to clone the gene of fructose binding protein BL0033 from Bifidobacterium longum NCC2705, and express and purify fusion protein GST-BL0033 in e.
另有两株为517和518、526和517位双位点突变,因为芯片上未点517位突变的探针,所以只检测到518和526位的突变,该突变与测序结果完全一致。
Because the probe of 517 rifampin-resistant did not be dotted on the gene chip, only sites of 518 and 526 mutations were detected, which were consistent with the results of DNA sequencing.
从健康婴儿粪便中分离出两株厌氧无芽孢杆菌,经鉴定为短双歧杆茵。
Two anoxic non-spore bacillus strains isolated from baby feces were identified as B. breve.
对流感样患者和鸡咽喉部采样,用常规鸡胚双腔法分离流感病毒并进行毒株鉴定。
The specimens for viral isolation were taken from throat of patients with influenza like disease, as well as from chickens, then the specimens were inoculated into embryonated chicken eggs.
对流感样患者和鸡咽喉部采样,用常规鸡胚双腔法分离流感病毒并进行毒株鉴定。
The specimens for viral isolation were taken from throat of patients with influenza like disease, as well as from chickens, then the specimens were inoculated into embryonated chicken eggs.
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