目的构建克隆载体，分析隐匿性乙型肝炎病毒S基因的突变情况，构建其原核融合蛋白表达载体。
Objective To construct the clone vector of S gene in occult hepatitis B virus infection. To analyse its mutations and to construct its prokaryotic expression vector.
目的构建凋亡素原核表达系统，以制备抗原物质凋亡素融合蛋白。
Objective to construct an apoptin expression system to produce an antigen, apoptin fusion protein.
结论：构建了8r - MUC1核心肽融合蛋白原核表达载体并成功表达与纯化出具有生物学活性的融合蛋白。
Conclusion the prokaryotic expression vector of 8r-muc1 core peptide fusion protein has been constructed, and the fusion protein with biological activity has been successfully expressed and purified.
细胞学观察表明，6一dmap阻止了纺锤体的形成和染色体的移动，导致一个融合的二倍性雄性原核的形成。
According to Cytological observations, 6-dmap disrupted the spindle at mitosis and inhibited chromosome movement, resulting in the formation of one diploid male nucleus.
结论利用原核表达体系成功地表达了不同分子质量的颗粒溶素融合蛋白，为颗粒溶素的后续研究奠定了基础。
Conclusion Different molecular weight granulysin were successfully expressed using prokaryotic expression system, which would be helpful for the further study of granulysin.
结论利用原核表达体系成功地表达了不同分子质量的颗粒溶素融合蛋白，为颗粒溶素的后续研究奠定了基础。
Conclusion Different molecular weight granulysin were successfully expressed using prokaryotic expression system, which would be helpful for the further study of granulysin.
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