原核生物的基因与真核生物的基因在组织形式和表达方式方面有哪些主要的区别？
What are the most significant differences between the organization and expression of prokaryotic genes and eukaryotic genes?
目的克隆人热休克蛋白72 (HSP72)基因，原核表达并纯化蛋白产物，以探讨其生物学功能。
AIM To clone human heat shock protein 72 (HSP72) gene, do prokaryotic expression and purify HSP72 protein for further study.
目的：克隆人MAGE3基因并进行原核表达和分离纯化。
AIM: to clone human MAGE 3 gene, to induce its prokaryotic expression and to purify the protein.
汪静。李官成。童永清鼻咽癌人源抗独特型基因工程抗体的原核表达及鉴定。
Wang J. Li GC Expression, purification and identification of the human anti-idiotypic single chain antibodies against nasopharyngeal carcinoma in E. coli.
目的克隆与原核表达“牵引丝蛋白基因”单体六聚物，为开展具有不同长度系列的“牵引丝蛋白基因”单体多聚物的功能研究奠定基础。
Objective To clone and express the hexamer of the gene of spider dragline silk protein, as a foundation of further research on the functions of the dragline silk protein gene of different lengths.
目的构建克隆载体，分析隐匿性乙型肝炎病毒S基因的突变情况，构建其原核融合蛋白表达载体。
Objective To construct the clone vector of S gene in occult hepatitis B virus infection. To analyse its mutations and to construct its prokaryotic expression vector.
结论原核表达系统可有效克隆较高纯度的CHM - I基因。
Conclusion The more effective CHM-I clone with high purity could be expressed by procaryotic expression system.
通过基因克隆等方法，选择原核表达系统，并对目的蛋白进行分离纯化，初步获得有活性的人内毒素结合肽ebp。
We isolated and purified EBP effectively, and obtained EBP with biological activity for the first time with gene cloning method and prokaryotic expression system.
目的：构建丙型肝炎病毒(HCV)全长及3种不同缺失突变的核心蛋白基因的原核表达载体，并在大肠杆菌中表达。
AIM: to construct the recombinant plasmids expressing full-length HCV core protein gene and 3 different deletion mutated hepatitis core protein genes and to express them in E. coli.
目的克隆人热休克蛋白70 (HSP70)基因，构建其原核高效表达载体。
Objective to clone human heat shock protein 70 (HSP70) gene for the construction of a prokaryotic expression vector.
我们利用基因重组技术构建了MGC5306的原核表达体系，并成功表达了MGC5306 (98- 204aa)。
We use gene recombination technology to build the prokaryotic MGC5306 expression system, and we have successfully expressed MGC5306 (98-204aa).
结论已成功构建了人hsp70与MAGE - 4抗原表位基因的原核表达载体，为疫苗研究提供了依据。
Conclusion the prokaryotic expression vector for HSP70 and MAGE-4 epitope genes was successfully constructed, which provided a basis for the development of vaccine.
目的改构高致病性禽流感病毒血凝素基因，建立有效的原核表达体系。
Objective To modify the HA1 gene of high pathogenic avian influenza virus (HPAIV) and to build an efficient prokaryotic expression system of it.
目的：构建食管癌相关基因2ECRG2原核表达质粒，在大肠杆菌e。
Objective: to construct a prokaryotic expression vector containing esophageal cancer related gene 2 ECRG2 and observe its expression in Escherichia coli e.
该载 体是一种环状载体，包含原核复制起点、真核复制起点、筛选标记基因和绿色荧光蛋 白基因表达盒；
The vector is in ring shape , comprising a procaryon replication origin, two eucaryon replication origins and selective marker genes, and two green fluorescent protein gene expression cassettes;
目的构建人鸟氨酸脱羧酶抗酶(OAZ)突变基因，重组至原核表达载体中并表达出重组蛋白。
Objective To clone human ornithine decarboxylase antizyme (OAZ) mutation gene and express its recombinant protein.
构建牛结核分枝杆菌esat - 6基因的原核表达载体，诱导表达、纯化并初步鉴定该蛋白。
To construct the recombinant prokaryotic expression vector containing the ESAT-6 gene of Mycobacterium Boris, purification, expression the fusion protein identified.
目的克隆人颗粒溶素基因并进行原核表达。
Objective To obtain recombinant human granulysin using prokaryotic expression system.
利用序列分析技术克隆与抗逆相关的基因，并通过原核表达体系和转基因技术进行功能验证。
The function of gene has been identified using Prokaryotic expression system and Arabidopsis thaliana (A. thaliana) transformation systems. The study has got following results:1.
利用序列分析技术克隆与抗逆相关的基因，并通过原核表达体系和转基因技术进行功能验证。
The function of gene has been identified using Prokaryotic expression system and Arabidopsis thaliana (A. thaliana) transformation systems. The study has got following results:1.
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