方法采用酶联免疫吸附法对40例特发性肺纤维化患者和30例健康人血清基质金属蛋白酶- 9进行测定。
Methods The serum level of MMP-9 in 40 patients with IPF and 30 healthy subjects was measured by ELISA.
主要观察指标：形态观察、增值率测定、碱性磷酸酶染色、细胞基质钙含量测定、茜素红染色显示矿化结节数。
MAIN OUTCOME MEASURES: Morphological observation, determination of proliferation ratio, ALP stain, determination of matrix calcium accumulation and number of mineralized nodes with alizarin red stain.
用酶标记免疫吸附测定法测定大鼠血浆VEGF水平，免疫组化方法测定肺组织vegf表达。
Plasma VEGF level was measured with ELISA method, and VEGF expression in lung was detected with immunohistochemistry method.
测定了固定化乳酸脱氢酶的表观米氏常数和人血清中的L-乳酸。
The apparant Michaelis constant of immobilized LDH and L-lactate in human serum are determined.
方法细胞瞬时和稳定转染及氯霉素酰基转移酶和芳香族化酶活性测定。
Methods Transient and stable transfection experiments in cells and assays of chloramphenicol acetyltransferase activity and aromatase activity were used.
并测定了固定化脂肪酶的催化性能，确定了该固定化酶催化橄榄油水解的最适条件。
The catalytic activity of the entrapped lipase in hydrolysis process of olive oil was investigated and optimal, hydrolysis conditions were determined.
应用亲和酶标组化标记法和免疫组化ABC法，测定106例乳腺癌组织中ER、PR和DAKO—M1水平。
The expression of ER, PR and DAKO-M1 in 106 breast carcinomas were examined by bothaffinitive histochemical and ABC technique.
方法采用美国标准化委员会的标准化文件EP10 T2 ，选择具有代表性的丙氨酸氨基转移酶（ALT）、血清总蛋白（TP） ，采用酶法测定ALT ，双缩脲法测定TP。
Method U. S Standardization Com mission's standard file EP10-T2 was used to select representative ALT and TP. AL T was determined with the enzyme reaction, TP was determined with biuret reaction .
方法：采用一步酶法测定血清中MMP -2的表达量，用免疫组化法检测MMP - 2在实体瘤中的表达情况。
Methods: The level of MMP 2 in serum was detected by a step sandwich enzyme immunoassay technique.
方法：采用免疫组化技术和高效液相-电化学法（ HPLC - EC）分别对酪氨酸羟化酶（TH）、DA进行了含量测定。
Methods: Immunohistochemistry technology and high-pressure liquid chromatography with electrochemical detector (HPLC-EC) were used to evaluated tyrosine hydroxylase (TH) and DA .
然后测定固定化酶的固载率，游离酶和固定化酶的活性，从而确定固定化酶的部分性质。
And then try to exam the immobilization yield of the immobilized enzyme, and the enzyme activities of immobilized enzyme and free enzyme to demonstrate some characters of immobilized enzyme.
免疫组化测定主动脉窦部MMP-2的表达；明胶酶谱法测定MMP-2的活性。
The expression and activation of matrix metalloproteinase-2 (MMP-2) were determined by the method of immunohistochemistry.
将此突变种固定化在聚氨脂泡沫中后，定量测定其谷氨酰胺合成酶(GS)活性。
After immobilized, the cells of the mutant within polyurethane (PU) foams, glutamine synthesize (GS) and NH4 + secretory activity of GS, and its growth and photosynthesis were measured.
测定了降解途径中相关酶的活性，表明对氯苯胺经过苯胺双加氧酶初始氧化和羟基化后，芳环的裂解是由邻苯二酚2 ，3双加氧酶催化。
Enzymatic analysis show that initial reactions ofp-chloroaniline degradation by Diaphorobacter sp. PCA039 are catalyzed by aniline dioxygenase and chlorocatechol 2,3-dioxygenase.
测定了降解途径中相关酶的活性，表明对氯苯胺经过苯胺双加氧酶初始氧化和羟基化后，芳环的裂解是由邻苯二酚2 ，3双加氧酶催化。
Enzymatic analysis show that initial reactions ofp-chloroaniline degradation by Diaphorobacter sp. PCA039 are catalyzed by aniline dioxygenase and chlorocatechol 2,3-dioxygenase.
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