在植物基因工程中，农杆菌质粒介导的遗传转化是比较完善与有效的基因转移方法，但在单子叶植物上应用较少。
The gene transformation by agrobacterium'plasmid is a perfect and effective method of transferring gene in the gene engineering, however, there is a little applied on the monocotyledons plants.
白菜遗传转化技术中最常用的方法是农杆菌介导法。
The common method of Genetic Transformation of Brassica campestris L. is Agrobacterium-mediated method.
介绍农杆菌介导的花生遗传转化的原理和方法并对影响转化率的因素进行了探讨。
The review introduced the theory and methods of Agrobacterium-mediated transformation of peanut, and the factors influencing transformation efficiency were discussed.
应用花生胚小叶再生体系，以农杆菌介导法对质粒上的基因转化进行初步探讨。
Applying to leaflet regeneration system of peanut, genetic transformation of peanut using Agrobacterium-mediated approach were carrying on the research.
目前，农杆菌介导法是苹果上应用的主要转化方法，叶片再生不定芽途径是被广泛应用的受体系统。
At present, Agrobacterium mediated transformation of leaves are widely adopted in apples, and transgenic shoots were mainly obtained from adventitious bud regeneration.
由于白菜的再生能力较低，因此农杆菌介导白菜遗传转化的研究报道较少。
The reports of this method in Brassica campestris L. can't often be seen because of its low shoot regeneration ability.
用农杆菌介导的叶盘转化法将一个来自棉花的谷胱甘肽s转移酶(GST)基因转化到烟草中。
A glutathione S-transferase (GST) gene from cotton was introduced into tobacco by Agrobacturium-mediated leaf disk transformation method.
其中以开花初期的植株，利用胭脂碱型农杆菌介导(如c58)，真空渗入5分钟的处理方法转化效率最高。
It was concluded that using the nopaline type of Agrobacterium (e. g. C58), 5min vacuum infiltration, and using the plants with just several blooming flowers would give the best results.
通过对影响根癌农杆菌介导蓝猪耳转化效果各种因素的研究，建立了蓝猪耳高效稳定的遗传转化系统。
An efficient and stable Agrobacterium-mediated genetic transformation system for Torenia was established through investigating the factors influencing the transformation efficiency.
目前我国在甘蔗遗传转化研究中应用的研究方法主要是农杆菌介导及基因检转化。
The genetic transformation methods for sugarcane in China are mainly Agrogacterium-mediated transformation and gene gun transformation.
以农杆菌介导的叶圆盘法将HAL1基因转化烟草植株。
HAL1 gene was transformed into tobacco plants and the screened transgenic plants showed improved salt tolerance than the control plants.
在植物基因工程中，农杆菌质粒介导的遗传转化是比较完善与有效的基因转移方法，但在单子叶植物上应用较少。
The gene transformation by agrobacterium'plasmid is a perfect and effective method of transferring gene in the gene engineering, however, there is a little apply on the monocotyledons plants.
优化农杆菌介导法转化棉花的主要技术参数，拓宽了棉花基因型范围，建立了四个棉花品种的高效遗传转化体系。
The main technical parameters were improved, the genotypes of cotton acceptors were widened, and a high efficient system of transformation of four varieties was established.
目前用于油菜基因转化的方法有根癌农杆菌介导法、基因枪法、PEG法、激光微束穿刺法、显微注射法和花粉介导法等。
At present, there are several methods of transformation in rape, including tumefaciens-medidated genetic etc, particle gun, PEG untake, vialaser microbeam puncture and microinjection.
目前用于油菜基因转化的方法有根癌农杆菌介导法、基因枪法、PEG法、激光微束穿刺法、显微注射法和花粉介导法等。
At present, there are several methods of transformation in rape, including tumefaciens-medidated genetic etc, particle gun, PEG untake, vialaser microbeam puncture and microinjection.
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