目的探讨在不同的免疫学检测方法中,与单链抗体相连的亲和标记肽对单链抗体与抗原结合活性的影响。
Objective to investigate the affects of affinity tag on binding activity of single chain variable fragment (ScFv) to antigen using different immunological assays.
过去主要是通过亲和标记和点突变等实验方法进行研究。
In the past, the Affinity Labeling and Site-Directed Mutagenesis are the primary methods to study amine receptor.
标记亲和多肽片段以其优越性在基因工程中占有重要的地位。
Affinity peptide (Marker) became more important in the development of genetic engineering.
在非变性条件下,用TALON金属亲和层析树脂纯化含组氨酸标记的重组DHFR。
The recombinant DHFR protein containing his-tag was purified by using TALON metal affinity resin under nondenaturing conditions.
应用亲和酶标组化标记法和免疫组化ABC法,测定106例乳腺癌组织中ER、PR和DAKO—M1水平。
The expression of ER, PR and DAKO-M1 in 106 breast carcinomas were examined by bothaffinitive histochemical and ABC technique.
方法:采用链酶亲和素-过氧化物酶标记免疫组织化学方法,在PCNA阳性标本中测定增殖指数(PI)。
Methods the PCNA index (PI) was detected by the immunohistochemistry method of streptavidin in the PCNA positive samples.
本文报道牛大脑突触膜 上苯二氮受体(BDZ-R)的光亲和标记性质。
This paper reports the properties of photoaffinity labeling of benzodiazepine receptors (BDZ-R) of bovine cerebral cortex.
采用链霉亲和素包被磁纳米粒子,将生物素标记的特异性抗体偶联在磁纳米粒子上,制备出高特异性的磁纳米探针;
The magnetic nanoparticle probe was prepared by specifically connecting the streptavidin-conjugated magnetic nanoparticles and the antibody of analyte via the strong streptavidin-biotin interaction.
采用链霉亲和素包被磁纳米粒子,将生物素标记的特异性抗体偶联在磁纳米粒子上,制备出高特异性的磁纳米探针;
The magnetic nanoparticle probe was prepared by specifically connecting the streptavidin-conjugated magnetic nanoparticles and the antibody of analyte via the strong streptavidin-biotin interaction.
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