Conclusion The influenza virus RNA polymerase PB1 1 is subcloned and expressed in E. coli successfully.
结论成功地亚克降及表达了流感病毒RNA聚合酶PB 1 - 1亚基。
Diagnostic confirmation using antibody tests, though reliable, is thus slower than that achieved using direct tests for virus RNA.
利用抗体试验进行诊断确认虽然可靠,但比利用对病毒RNA直接检测取得的诊断确认更为缓慢。
Methods the 64 serum samples were collected from patients of hemorrhagic fever with renal syndrome (HFRS), and the virus RNA was extracted as pattern.
方法采集64例肾综合征出血热(HFRS)急性期患者血清标本,提取血清中病毒RNA作为模板。
Objective To establish a super-sensitive diagnosis method for influenza a (H1N1) by enrichment of virus RNA using several settling agents and nucleic acid purification methods.
目的利用几种助沉剂和核酸纯化方法富集病毒rna,建立甲型H1N 1流感超敏感诊断方法。
The virus RNA without purification could be detected with ELISA technique, which is simpler and rapider than RT-PCR technique, and is more suitable for the detection of a great number samples.
ELISA可在不需提纯病毒RNA的情况下实现对病毒的检测,较RT - P CR检测技术快速、简捷,更适合于大量样品的检测。
The virus is a single-stranded RNA virus belonging to the virus family Arenaviridae.
该病毒是一种单链核糖核酸(RNA)病毒,属于沙粒病毒科。
Sat-RNA of CMV is a subviral agent, which depends on its helper virus to replicate and cell to cell diffuse.
CMV的 Sat-RNA是一种亚病毒剂,取决于其辅助病毒的复制和细胞间的扩散。
Once inside the cell, if the RNA computer finds tracers of a virus or cancer it could release the appropriate drugs to kill just that one cell.
RNA计算机一旦进入某一细胞内部,如果发现病毒或癌症的踪迹,它就可以释放相应药物来仅仅杀死这一个细胞。
In monkeys treated with minocycline, the virus load in the cerebrospinal fluid, the viral RNA in the brain and the severity of central nervous system disease were significantly decreased.
在给予二甲胺四环素的猴子中,病毒装载进入脑脊液,病毒RNA在脑部以及中枢神经系统疾病的严重性降低。
During RNA replication - the phase during which the virus "reproduces" - all three of the subunits of the protein assemble themselves in a particular configuration.
RNA复制期间即病毒处于增值期蛋白质的所有亚单位组装成一个特殊的结构。
These structures contain short nucleotide sequences that find the most conservative molecules among all RNA molecules, that is, sequences that do not change quickly and are important to the virus.
这些片断中包含在所有RNA分子中最为稳定的短核苷酸序列,这种RNA序列变化缓慢且对病毒很重要。
In H5N1, perhaps the most important of these proteins is RNA polymerase, which contains the instructions that allows the virus to copy itself along with all of its genetic material.
H5N 1中最重要的蛋白质可能是RNA聚合酶,它使病毒以所有自身基因物质而完成自我复制。
RNA polymerase in RNA virus is different from host cells restrict it's high and early expression. The restrict is moderate compared with other virus genes.
RNA病毒的聚合酶基因总体上和宿主密码子使用类型不一致,限制了聚合酶基因的及早和过高表达,但其密码子使用频率对聚合酶的限制是适中的。
Using RNA virus as vaccine vector are becoming hotspots in the field of vaccine research these years for their prominent advantages such as easy manipulation and high efficiency.
RNA病毒作为疫苗载体,在可操作性和免疫效果方面有着显著的优势,近年来已成为疫苗研究领域的热点。
A complete viral particle consisting of RNA or DNA surrounded by a protein shell and constituting the infective form of a virus.
病毒粒子一整个病毒粒子,由被蛋白质外壳包围的核糖核酸或脱氧核糖核酸组成,并构成一个病毒的传染形式。
Viruses that can be modified include stranded RNA viruses, especially plant stranded RNA viruses such as the cowpea Mosaic virus.
可被修饰的病毒包括链rna病毒,特别是植物链rna病毒,例如豇豆花叶病毒。
Conclusions: a specific RT_PCR assay was established for detecting the genomic RNA of WEE virus from infected Suckling mice and culture cells.
结论:所建立的RT _PCR方法可自病毒感染的小鼠脑组织和传代细胞中检测WEE病毒的基因组rna。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR product was cloned into t vector, sequenced and analyzed using DNASTAR software.
方法从Z 10株病毒感染的细胞提取总rna,将逆转录pcr扩增的产物纯化后克隆于t载体并进行序列测定,应用dnastar软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR products was cloned into t vector, sequenced and analyzed by using DNASTAR software.
方法从Z 10病毒感染的细胞提取总rna,将逆转录聚合酶链反应(RT PCR)扩增的产物纯化后克隆于t载体并进行序列测定,应用dnas TAR软件分析比较。
Objective To explore the effect of antisense RNA on hepatitis B virus (HBV) replication and antigen synthesis.
目的了解反义乙型肝炎病毒(HBV)基因转移、表达的抗HBV作用。
HCV is a single - stranded positive sense RNA virus, the whole genome displaying a higher degree of genetic variability.
HCV为一单股正链r NA病毒,整个基因组具有高度变异性。
Lactoferrin displays antiviral activity against HIV, both DNA - and RNA-viruses, including rotavirus, respiratory syncytial virus, and herpes viruses.
乳铁蛋白表现出抗人免疫缺陷病毒(HIV)、抗rna和DNA病毒、包括呼吸道合胞体病毒和疱疹病毒等。
So the homology of relevant nucleic acid and amino acid sequence were compared and analyzed with that of AEV 1143 strain and others of small RNA virus family.
并将其与已发表的AEV 1143株相关基因以及小rna病毒科其它病毒属在对应位置的核苷酸和氨基酸的同源性进行比较、分析。
The primary function of the influenza virus nucleoprotein (NP) is to encapsidate the virus genome for the purposes of RNA transcription, replication and packing.
流感病毒核蛋白(NP)的主要功能是使病毒基因组衣壳化,以便RNA转录、复制和病毒子装配。
Methods the total RNA was extracted from Z37 virus infected cells and the RT-PCR products were cloned into t vector, sequenced and analyzed by DNASTAR software.
方法设计出针对各片段的特异性引物,用P CR方法从Z 37病毒感染的细胞提取细胞总rna,逆转录扩增、产物克隆t载体,纯化后测序,测定的序列应用DNASTAR软件比较分析。
Methods the total RNA was extracted from Z37 virus infected cells and the RT-PCR products were cloned into t vector, sequenced and analyzed by DNASTAR software.
方法设计出针对各片段的特异性引物,用P CR方法从Z 37病毒感染的细胞提取细胞总rna,逆转录扩增、产物克隆t载体,纯化后测序,测定的序列应用DNASTAR软件比较分析。
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