Gene transfection is the key technique of gene therapy.
基因治疗的关键技术是基因转移。
The application prospect of Gene pulser transfection is very extensive.
基因脉冲导入技术应用前景十分广泛。
Albumin, serum in the culture medium decreased the transfection efficiency.
培养液中的白蛋白、血清可降低转染效率。
Microbubbles at concentration of 10% led to the highest transfection effect.
微泡浓度为10％时细胞转染效率最高。
However, low genetic transfection efficiency limits their clinical application.
然而低转染效率限制了其在临床上的应用。
Objective: To optimize the condition of gene transient transfection in CHO-K1 cell.
目的：以CHO - K1细胞为宿主基因瞬时转染条件的优化。
The gene transfection technology might be one of the approaches to resolve these issues.
应用基因转染技术可能是解决这些问题的途径之一。
To explore the effects of IL-18 gene transfection on the proliferation of C6 glioma cell.
探讨IL-18基因转染对大鼠C6胶质瘤细胞生长特性的影响。
Objective To optimize the transfection rate in COS 7 cell line with electroporation method.
目的以电穿孔法转染COS7细胞系以得到较高的转染率。
To investigate the effects of lentivirus-mediated transfection of GDNF on Parkinson's disease.
探讨慢病毒介导GDNF对帕金森病的治疗作用。
The result of experiment indicates that the Gene Transfection Apparatus is good for curing tumor.
实验结果表明：基因脉冲导入仪有利于肿瘤的治疗。
By G418 screening and colonies counting, gene pool A was chosen for the second cycle transfection.
经G418筛选并计集落数，选择克隆数最多的基因池进入第二轮。 如此反复进行。
Conclusions: The growth of tumor can be inhibited significantly by antisense-VEGF gene transfection.
结论反义vegf基因转染可以有效地抑制喉癌的生长。
Objective To study the effect of RAB5A gene transfection on the invasion of colorectal neoplasm cells.
目的研究RAB5A基因转染后对大肠癌细胞侵袭能力的影响。
Additionally, PEI is considered to exhibit high transfection efficiency and endosomal disrupting capacity.
此外，PEI也被认为具有高转染率和使内涵体破裂的能力。
Protection of exogenous genes against intracellular conditions is one of key factors in gene transfection.
外源基因在细胞内的酶切保护问题是影响基因转染成败的重要因素之一。
Flow cytometry revealed that E1A transfection induced s stage suppression and G2 stage arrest in Hela cells.
流式细胞术结果显示：转染e1 A基因后，细胞周期出现S期抑制，G 2期阻滞。
After gene transfection, GH3 cell specifically expressed reporter gene and killed specifically by therapy gene.
基因转染后，GH 3细胞特异性表达报告基因，并被治疗基因特异性杀伤。
CONCLUSION Lipofectamine may be used as a tool to enhance the efficacy of transfection with recombinant adenovirus.
结论可以利用阳离子脂质体来提高重组腺病毒的转染效率。
The operation temperature and the concentration of serum in buffer have no effect on the transfection efficiencies.
温度和缓冲液中的血清成分对电穿孔效率影响不大。
Objective To study the effects of transfection of tyrosine kinases on the tumorigenesis of nasopharyngeal carcinoma.
目的：研究酪氨酸激酶转染对鼻咽癌细胞成瘤的影响。
For rapid establishment of mouse thymic stromal cell(MTSC) lines, gene transfection was used to immortalize fresh viable cells.
利用基因转染方法使细胞获得不死性，快速建立小鼠胸腺基质细胞（MTSC）系。
OBJECTIVE To study the effect of the lipid compositions on cellular uptake and gene transfection mediated by cationic liposomes.
目的研究阳离子脂质体膜的组分对细胞摄取和基因转染的影响。
Conclusion: Transfection of VEGF165 gene enhances local angiogenesis in early time and DPB-VEGF compound improves bone formation.
结论：VEGF165基因转染可促进局部血管的早期形成，DPB - VEGF复合物可增加骨形成。
Proliferation suppression and apoptosis of 8348 cells pre and post transfection induced by cisplatin were analysed by MTT methods.
用MTT法分析顺铂对转染前后的8348细胞抑制增殖和诱导凋亡的能力。
DNA condensation is a common physiological phenomenon in nature and also a key step in non-viral vector-mediated gene transfection.
DNA缩合不仅是自然界常见的生理现象，也是非病毒载体介导基因转染的关键步骤。
After 2 weeks of gene transfection, the femoral arteries of the rats were excised to establish the model of left hind-limb ischemia.
基因转染2周后行股动脉切除术建立右侧大鼠后肢缺血模型。
Objective: To explore whether transfection of osteosarcoma cells with B7-1 gene can induce anti-osteosarcoma active immune response.
目的：探讨B7 - 1基因转染骨肉瘤细胞能否诱导抗骨肉瘤主动免疫作用。
Objective To explore the efficiency of transfection of antisense oligonucleotides HA-2741 by ultrasound mediated liposome microbubbles.
目的探讨超声介导脂质体微气泡转染反义寡核苷酸ha- 2741的有效性。
Objective To explore the efficiency of transfection of antisense oligonucleotides HA-2741 by ultrasound mediated liposome microbubbles.
目的探讨超声介导脂质体微气泡转染反义寡核苷酸ha- 2741的有效性。
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