Objective To isolate CD4+ T lymphocyte subpopulation by antibody-coating immunomagnetic beads and evaluate the efficacy of detective methods.
目的应用抗体包被免疫磁珠分离CD4 +T淋巴细胞亚群，并检测其效果。
Magnetic absorption cell sorting (MACS) method was used to isolate the CD4 + t lymphocytes from the spleen of the rats. Flow cytometry was used to monitor the efficiency of isolation.
利用磁吸附细胞分选法(MACS)分离大鼠脾脏CD 4 +T细胞，流式细胞术检测MACS分选前后CD 4 + T细胞比例，监测细胞分选效果。
Conclusion Direct antibody coating of immunomagnetic beads can isolate CD4 + t lymphocyte subpopulation effectively.
结论用直接包被法制备的免疫磁珠具有较好的分离CD 4 +T淋巴细胞亚群效果。
The objective of the present study was to isolate Toxoplasma gondii strains from cats and then to identify these strains by specific PCR assay. The T.
将猫的心、脑、舌用盐酸-胃蛋白酶溶液消化处理后接种小白鼠，经连续传代分离弓形虫虫株，用特异PCR方法对所分离的虫株进行了鉴定。
Methods Isolate MSCs from CML patients and normal donors respectively and exam their differences on the t lymphocyte proliferation by MLR, on the t lymphocyte cycle and apoptosis using flow cytometry.
方法分离正常人和CML患者的骨髓中的MSC,MLR法检测其对T细胞增殖的影响，流式检测其对对T细胞周期、凋亡的作用情况。
Methods Isolate MSCs from CML patients and normal donors respectively and exam their differences on the t lymphocyte proliferation by MLR, on the t lymphocyte cycle and apoptosis using flow cytometry.
方法分离正常人和CML患者的骨髓中的MSC,MLR法检测其对T细胞增殖的影响，流式检测其对对T细胞周期、凋亡的作用情况。
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