Methods: PCR, gene cloning and successive sub cloning, DNA sequencing, prokaryotic temperature induction, etc. were used.

方法：采用PCR，克隆及连续亚克隆，序列分析，原核温度诱导表达等方法。

youdao

Methods: PCR, gene cloning and successive sub cloning, DNA sequencing, prokaryotic temperature induction, etc. were used.

方法：采用PCR，克隆及连续亚克隆，序列分析，原核温度诱导表达等方法。

youdao