• No variant shift bands were found by SSCP.

    SSCP分析未发现变异迁移

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  • And analyze the amplification products with SSCP.

    扩增产物进行SSCP分析。

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  • Results The SSCP analysis showed false positive results.

    结果SSCP分析存在阳性结果。

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  • The point mutations in these fragments were detected by SSCP.

    单链构象多态性对扩增片段进行突变检测。

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  • Methods Make a comparison between PCR SSCP and immunohistochemical method.

    方法应用pcrSSCP免疫组织化学方法对比研究。

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  • The results of gene array corresponded with that of PCR-SSCP and DNA sequencing.

    基因阵列检测结果PCR SSCP测序结果一致。

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  • Methods:Polymerase chain reaction (PCR) and single strand conformation polymorphism(SSCP) was used.

    方法聚合酶链反应PCR)、单链构象多态性SSCP)分析。

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  • The SSCP electrophoresis lanes of all strains were incongruence, and some strains had several conformation.

    各条带之间显现出一定差异性有部分菌株的单链具有多种构象

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  • The CD4-cell count was not significantly correlated with the number of SSCP bands, p-distance, HCV viral load.

    免疫指标CD4+细胞数种数、核苷酸差异度、HCV病毒载量之间也没有显著相关性

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  • Method Exon 17 of RET proto oncogene from 30 children with Hirschsprung's disease was detected by PCR SSCP method.

    方法应用PCRSSCP银染技术30例先天性巨结肠癌基因RET第17外进行检测。

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  • Sequence variation was screened by means of PCR single stranded conformation polymorphism (SSCP) and DNA sequencing.

    PCR -单链构象多态性(SSCP)DNA序列分析方法确定扩增序列突变情况。

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  • This result showed that PCR-SSCP analysis could be effectively used for the direct gene diagnosis of phenylketonuria.

    SSCP分析法有效地用于苯丙酮尿基因诊断

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  • Results After analyzing the band position of 198 multiclones in SSCP electrophoresis, 58 clones was selected to sequence.

    结果198个克隆SSCP电泳条带位置分析,挑选58个克隆测序

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  • It was concluded that PCR SSCP silver staining method for the detection of P53 gene mutation is quick reliable and practical.

    结论:PCRSSCP染色一种可靠快速、实用P 53基因突变检测方法。

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  • Conclusions Appropriate LPA concentration, running temperature and running voltage can improve ce performance in SSCP analysis.

    结论:调整适宜的LPA浓度、分离温度分离电压提高CE分析SSCP的效率。

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  • It was reported that glycerol in the non-denatured SSCP polyacrylamide gel could increase the sensibility of detecting mutation.

    有文献报道非变性聚丙烯酰胺加入甘油提高SSCP检测灵敏度

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  • The Polymorphisms of IL 10 Promoter region were detected by PCR SSCP and sequencing. 66 of health control were examined by this way.

    聚合酶链反应单链构象多态性( PCR-S SCP)结合序列测定方法,检测了66例健康对照I L-10启动子多态性发生。

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  • Objective: To quickly detect two pathogenic bacteria strains of food poisoning by single strand conformation polymorphism (SSCP) assay.

    目的:利用单链构象多态性(SSCP)对食物中毒致病菌进行快速分析

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  • Single strand conformation polymorphism (SSCP) essay and sequence analysis of the PCR product were used to ascertain the gene mutation.

    方法应用PCR单链构象多态性(SSCP)分析技术结合基因序列测定方法确定突变类型。

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  • Objective:To assess the roles of single stranded conformation polymorphism(SSCP)and heteroduplex polymorphism(HET)in screening gene mutation.

    目的评价异源双链分析(HET)单链构象多态性分析(SSCP基因突变检测中的作用

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  • Methods:Mutations of multiple genes from 106 patients with SCC were analyzed by two multiplex PCR-SSCP systems, PCR-RFLP, and DNA sequencing.

    方法:对10 6例散发性大肠癌患者进行基因突变和肠道内环境中有关指标(以粪便为标本)的测定,并进行流行病学的病例-病例研究分析

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  • Objective: To observe the effect of different gel preparation on detecting mutation of AKT2 gene by single-strand conformation polymorphism (SSCP).

    目的观察单链构象多态性(SSCP)筛查akt2基因突变时凝胶配制不同对筛查结果的影响

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  • PCR and single strand conformation polymorphism analysis (SSCP) were combined with DNA sequencing confirmation to screen all 28 exons of SCN5A gene.

    采用PCR单链构象多态性技术(SSCP结合DNA序列测定证实病人SCN5A全部2 8个外子进行突变检测。

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  • One hundred and twenty two Nanyang cattle were used for SNPs discovery in the complete coding region of HCRTR1 gene using PCR-SSCP and sequencing methods.

    研究南阳HCRTR1基因编码核苷酸多态性,利用遗传标记进行肉牛选育奠定基础

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  • Using PCR-SSCP and PCR product direct sequencing techniques, we analysed the ORF region of SOX4 gene encoding in 15 lung cancer tissues and 8 normal controls.

    本文采用PCR-SSCPPCR产物直接测序等技术,对15肺癌患者组织标本8例正常对照个体血细胞SOX4基因编码区进行了突变分析

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  • Methods: Using PCR SSCP, we detected the mutation of INSR tyrosine kinase domain exon 17 and exon 18 in 33 primary hypertension patients and 28 normal persons.

    方法:对原发性高血压伴胰岛素抵抗患者胰岛素受体基因酪氨酸激酶域外1718基因突变进行检测,并对突变的外显子进行核苷酸序列分析。

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  • The polymorphism of the insulin-like growth factor binding protein 3 (IGFBP3) gene in Nanyang cattle, Luxi cattle and Chinese Simmental was analyzed by PCR-SSCP.

    采用PCR-SSCP技术分析了类胰岛素生长因子结合蛋白3IGFBP3基因南阳牛、鲁西中国西门塔尔牛3个牛品种中的遗传多态性。

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  • Methods:Immunohistochemistry and PCR-SSCP were used to detect the expression of P53 protein and p53 gene mutation in 32 cases of TSCC and 10 cases of tongue leukoplakia.

    方法应用免疫组织化学聚合酶链反应—单链构象多态性分析(PCR-SSCP检测10白斑32癌,结合临床资料进行分析。

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  • Methods:Immunohistochemistry and PCR-SSCP were used to detect the expression of P53 protein and p53 gene mutation in 32 cases of TSCC and 10 cases of tongue leukoplakia.

    方法应用免疫组织化学聚合酶链反应—单链构象多态性分析(PCR-SSCP检测10白斑32癌,结合临床资料进行分析。

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