No variant shift bands were found by SSCP.
SSCP分析未发现变异迁移带。
And analyze the amplification products with SSCP.
并对扩增产物进行了SSCP分析。
Results The SSCP analysis showed false positive results.
结果:SSCP分析存在假阳性结果。
The point mutations in these fragments were detected by SSCP.
用单链构象多态性对扩增片段进行点突变检测。
Methods Make a comparison between PCR SSCP and immunohistochemical method.
方法:应用pcrSSCP及免疫组织化学方法对比研究。
The results of gene array corresponded with that of PCR-SSCP and DNA sequencing.
基因阵列检测结果与PCR SSCP及测序结果一致。
Methods:Polymerase chain reaction (PCR) and single strand conformation polymorphism(SSCP) was used.
方法:聚合酶链反应(PCR)、单链构象多态性(SSCP)分析。
The SSCP electrophoresis lanes of all strains were incongruence, and some strains had several conformation.
各条带之间都显现出一定的差异性,有部分菌株的单链具有多种构象。
The CD4-cell count was not significantly correlated with the number of SSCP bands, p-distance, HCV viral load.
免疫指标CD4+细胞数与准种数、核苷酸差异度、HCV病毒载量之间也没有显著相关性。
Method Exon 17 of RET proto oncogene from 30 children with Hirschsprung's disease was detected by PCR SSCP method.
方法应用PCRSSCP银染技术对30例先天性巨结肠原癌基因RET第17外显子进行检测。
Sequence variation was screened by means of PCR single stranded conformation polymorphism (SSCP) and DNA sequencing.
用PCR -单链构象多态性(SSCP)和DNA序列分析方法确定扩增序列突变情况。
This result showed that PCR-SSCP analysis could be effectively used for the direct gene diagnosis of phenylketonuria.
SSCP分析法可有效地用于苯丙酮尿症的基因诊断。
Results After analyzing the band position of 198 multiclones in SSCP electrophoresis, 58 clones was selected to sequence.
结果对198个克隆的SSCP电泳条带位置分析,挑选出58个克隆测序。
It was concluded that PCR SSCP silver staining method for the detection of P53 gene mutation is quick reliable and practical.
结论:PCRSSCP银染色法是一种可靠、快速、实用的P 53基因突变检测方法。
Conclusions Appropriate LPA concentration, running temperature and running voltage can improve ce performance in SSCP analysis.
结论:调整适宜的LPA浓度、分离温度和分离电压可提高CE分析SSCP的效率。
It was reported that glycerol in the non-denatured SSCP polyacrylamide gel could increase the sensibility of detecting mutation.
有文献报道在非变性的聚丙烯酰胺中加入甘油可提高SSCP检测的灵敏度。
The Polymorphisms of IL 10 Promoter region were detected by PCR SSCP and sequencing. 66 of health control were examined by this way.
用聚合酶链反应和单链构象多态性( PCR-S SCP)结合序列测定方法,检测了66例健康对照I L-10启动子区多态性的发生。
Objective: To quickly detect two pathogenic bacteria strains of food poisoning by single strand conformation polymorphism (SSCP) assay.
目的:利用单链构象多态性(SSCP)对两起食物中毒的致病菌进行快速分析。
Single strand conformation polymorphism (SSCP) essay and sequence analysis of the PCR product were used to ascertain the gene mutation.
方法应用PCR及单链构象多态性(SSCP)分析技术结合基因序列测定方法确定突变类型。
Objective:To assess the roles of single stranded conformation polymorphism(SSCP)and heteroduplex polymorphism(HET)in screening gene mutation.
目的:评价异源双链分析(HET)和单链构象多态性分析(SSCP)在基因突变检测中的作用。
Methods:Mutations of multiple genes from 106 patients with SCC were analyzed by two multiplex PCR-SSCP systems, PCR-RFLP, and DNA sequencing.
方法:对10 6例散发性大肠癌患者进行多基因突变和肠道内环境中有关指标(以粪便为标本)的测定,并进行流行病学的病例-病例研究分析。
Objective: To observe the effect of different gel preparation on detecting mutation of AKT2 gene by single-strand conformation polymorphism (SSCP).
目的:观察单链构象多态性(SSCP)筛查akt2基因突变时凝胶配制的不同对筛查结果的影响。
PCR and single strand conformation polymorphism analysis (SSCP) were combined with DNA sequencing confirmation to screen all 28 exons of SCN5A gene.
采用PCR单链构象多态性技术(SSCP)结合DNA序列测定证实,对病人SCN5A的全部2 8个外显子进行突变检测。
One hundred and twenty two Nanyang cattle were used for SNPs discovery in the complete coding region of HCRTR1 gene using PCR-SSCP and sequencing methods.
研究南阳牛HCRTR1基因编码区单核苷酸多态性,为利用遗传标记进行肉牛选育奠定基础。
Using PCR-SSCP and PCR product direct sequencing techniques, we analysed the ORF region of SOX4 gene encoding in 15 lung cancer tissues and 8 normal controls.
本文采用PCR-SSCP及PCR产物直接测序等技术,对15例肺癌患者的组织标本及8例正常对照个体血细胞SOX4基因编码区进行了突变分析。
Methods: Using PCR SSCP, we detected the mutation of INSR tyrosine kinase domain exon 17 and exon 18 in 33 primary hypertension patients and 28 normal persons.
方法:对原发性高血压伴胰岛素抵抗患者胰岛素受体基因酪氨酸激酶域外显子17、18基因突变进行检测,并对突变的外显子进行核苷酸序列分析。
The polymorphism of the insulin-like growth factor binding protein 3 (IGFBP3) gene in Nanyang cattle, Luxi cattle and Chinese Simmental was analyzed by PCR-SSCP.
采用PCR-SSCP技术分析了牛类胰岛素生长因子结合蛋白3(IGFBP3)基因在南阳牛、鲁西牛和中国西门塔尔牛3个牛品种中的遗传多态性。
Methods:Immunohistochemistry and PCR-SSCP were used to detect the expression of P53 protein and p53 gene mutation in 32 cases of TSCC and 10 cases of tongue leukoplakia.
方法:应用免疫组织化学和聚合酶链反应—单链构象多态性分析(PCR-SSCP)检测10例舌白斑和32例舌癌,并结合临床资料进行分析。
Methods:Immunohistochemistry and PCR-SSCP were used to detect the expression of P53 protein and p53 gene mutation in 32 cases of TSCC and 10 cases of tongue leukoplakia.
方法:应用免疫组织化学和聚合酶链反应—单链构象多态性分析(PCR-SSCP)检测10例舌白斑和32例舌癌,并结合临床资料进行分析。
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