Today we cross the Rubicon. There is no going back.
今天我们要破釜沉舟，背水一战了。
Mr Yanukovich, by contrast, seems to have waded across the Rubicon without noticing.
而亚努科维奇先生好像还不知道自己已经跨越了界限。
At last they came to a little river called the Rubicon.
最后，他们来到了一条叫做卢比孔的小河边。
Their entry into the war made them cross the Rubicon and abandon isolationism forever.
他们的参战使他们破釜沉舟永远弃绝孤立主义。
Ever since Julius Caesar crossed over that stream, "crossing the Rubicon" has meant making a life-changing decision.
自从凯撒过了这条小河后，“越过卢比孔河”这句话，便成了作一个改变一生的决定的意思。
Nevertheless, a Rubicon has been crossed. It is now possible to conceive of a world in which new bacteria (and eventually, new animals and plants) are designed on a computer and then grown to order.
但是这一步已然了迈出去，我们可以预见到将来我们会用电脑设计新的细菌(并且最终会出现新的动物和植物)，并让它按计划生长。
Nevertheless, a Rubicon has been crossed. It is now possible to conceive of a world in which new bacteria (and eventually, new animals and plants) are designed on a computer and then grown to order.
但是这一步已然了迈出去，我们可以预见到将来我们会用电脑设计新的细菌(并且最终会出现新的动物和植物)，并让它按计划生长。
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