METHODS: Whole cell patch-clamp recording techniques.
方法：全细胞膜片箝技术。
The automatic patch-clamp system had been analyzed and modeled by using the method of UML in RUP.
通过基于RUP的UML系统建模方法对全自动膜片钳软件系统进行了分析和建模。
Method The experiment was carried out in freshly isolated bullfrog using a whole-cell patch-clamp technique.
方法实验采用的方法是全细胞膜片钳技术，实验材料为急性分离的牛蛙背根神经节神经元。
The whole-cell patch-clamp recording technique was used to record the change of sodium channel current influenced by carvedilol.
方法用急性酶解法获得单个大鼠心室肌细胞，标准的全细胞膜片钳技术记录钠通道电流。
The visual patch-clamp method can accurately assess synaptic transmission by measuring postsynaptic currents of the dorsal horn neurons.
可视膜片钳方法可以准确测量脊髓背角神经元的突触后电流从而研究初级传入突触的传递过程。
Methods In acutely isolated rat DRG neurons, effects of loureirin B on capsaicin-evoked currents were observed using whole-cell patch-clamp technique.
方法采用全细胞膜片钳技术在急性分离的大鼠背根神经节细胞上观察龙血素B对辣椒素诱发的辣椒素受体电流的影响。
Methods Isolated mouse cardiac ventricular myocytes were subjected to whole-cell patch-clamp to record the hypotonicity activated chloride channel currents.
方法采用急性分离的小鼠心室肌细胞，低渗溶液激活容积敏感性氯通道电流，膜片钳全细胞记录法记录电流。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
方法传代得到单个HERG-HEK细胞，应用全细胞膜片钳技术记录HERG-HEK细胞上的HERG钾电流和动力学曲线（激活、失活、复活和去活化）。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
膜片钳技术是一种以记录离子通道的离子电流来反映细胞膜上单一的或多个的离子通道分子活动的技术。
The methods of isolation and primary cultivation of hippocampal pyramidal neurons of SD rats improved the growth of neurons and so might be used in patch-clamp recording.
表明本分离及原代培养方法，有益于大鼠海马锥体神经元生长，并适用于膜片钳记录。
As a result single guinea-pig atrial myocyte with normal electrophysiologic activity was obtained and its L-Ica and IK were successfully recorded with patch-clamp technique.
结果获得了具正常电生理活性的单个细胞及高阻封接的形成，成功记录了L -型钙流和延迟整流性钾流。
Results: (1) Whole-cell patch-clamp recording technique was established, and highly stable whole cell ion channels' current curves of normal ventricular myocytes were ploted.
结果：(1)建立了膜片钳全细胞记录技术平台，获得了高稳定性的正常心室肌细胞全细胞离子通道电流图。
The effects of streptomycin on ionic currents in outer hair cells isolated from the cochlea of guinea pig were investigated using a whole-cell recording patch-clamp technique.
使用全细胞式记录膜片钳技术，研究链霉素对豚鼠耳蜗外毛细胞离子电流的影响。
The first one clearly elucidates the operational principle of patch-clamp amplifier and the compensatory technology, which are the fundamental for fulfilling the automation of patch clamp.
首先对膜片钳放大器工作原理及补偿技术进行了深入研究，这是实现膜片钳自动化的基础。
Mechanical dissociation was used to obtain single primary spermatocytes from guinea pigs and iron channel currents in the primary spermatocytes were recorded by whole cell patch-clamp technique.
笔者应用机械分离获得豚鼠单个初级精母细胞，采用全细胞式膜片钳技术记录该细胞离子通道电流。
In traditional patch-clamp amplifiers, the parameters for capacitance compensation are manually configured according to the observation of experimenters, which is very complicated and discommodious.
传统膜片钳放大器中，电容补偿参数配置需手动完成，过程复杂、繁琐。
Using the whole-cell patch-clamp technique, clear evidence was provided for the existence of a hitherto unreported, voltage-dependent nonselective cation current (NSCC) in rabbit ventricular myocytes.
应用全细胞膜片钳制技术，我们首次报道了在兔的心室肌细胞存在一种电压依赖性的非特异性阳离子流（NSCC）。
Methods: Patch clamp whole cell recording technique was used.
方法：膜片钳全细胞电流记录方法。
Patch clamp technique is a kind of technique which reflecting membrane molecular activity of one or more ion channel by record ion current of ion channel.
膜片钳技术是一种以记录离子通道的离子电流来反映细胞膜上单一的或多个的离子通道分子活动的技术。
METHODS Patch clamp technique with whole cell configuration.
方法膜片钳制技术全细胞记录模式。
METHODS The patch clamp whole cell recording technique was used.
方法采用膜片钳全细胞式记录技术。
The widely using of patch clamp technique has greatly promoted our knowledge of ion channels.
膜片钳技术的广泛应用极大推动了人们对离子通道的认识。
METHOD The whole cell patch clamp techniques were used.
方法采用全细胞膜片钳技术。
METHODS Voltage gated potassium outward current was recorded using whole cell patch clamp techniques in the cultured rat hippocampal neurons.
方法在培养的大鼠海马神经元上用膜片钳全细胞记录钾通道电流。
In patch clamp experiments, for measuring the activities of live cells in various physiological environments, it is indispensable to perfuse a wide range of drugs to live cells.
膜片钳实验中，为了检测细胞在不同的生理环境下的生物活性，需要向活体细胞施加各种不同的药品。
Methods Effects of quinidine and verapamil on the alteration of action potential duration induced by aconitine were studied by use of the whole cell patch clamp techniques in rat ventricular myocytes.
方法应用全细胞膜片钳技术记录乌头碱对大鼠心肌细胞动作电位时程的作用，分别给予奎尼丁、维拉·帕米处理比较二者对动作电位时程的影响。
METHODS The whole cell patch clamp techniques were used.
方法全细胞膜片钳技术。
Method The whole cell patch clamp method was used.
方法应用全细胞膜片钳制技术。
Methods: the whole cell patch clamp technology was used to record this current and study its change under the condition of hypoxia-reoxygenation model.
方法：运用全细胞膜片钳技术记录持续性钠电流，并观察其在缺氧-复氧模型下的变化。
Methods: The patch clamp was used to record the changes in average whole cell current and the conductance of GABA-A receptor on cultured hippocampal neurons in different developing stages.
方法用膜片钳记录仪在体外培养的不同时期，记录大鼠海马神经元GABA-A受体离子通道全细胞通道电流、电导的变化。
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