One of the things about these plasmids that makes them very useful is that their entire base pair sequences is known.
质粒之所以有用,其中一个原因就是,它的全部碱基序列都是已知的
That means the gene that encodes insulin, the sequence of base pairs that encode insulin.
这意味着基因编码了胰岛素,碱基序列编码了胰岛素
I just give you one of those, right, because you could write down the other one because you know the other complimentary sequence from base pairing?
我只写了一条链的序列,因为你们能够写出另一条,因为你们根据碱基配对,就能推测出互补链的序列
They face in the opposite direction and you could predict the properties of one from base pairing rules of the other but they're not the same.
它们是反向的,尽管你能够通过一条链上的碱基序列,预测另一条链的序列,但这两条链是不同的
They do that in a very special way in that they - restriction enzymes are able to identify a particular sequence of bases in a gene.
限制性内切酶的工作机制很特别,它能识别基因中的一段特定的碱基序列
If you do the base pairing goes exactly the same sequence backwards down here.
如果将它碱基配对,就会得到相同,但反过来的序列
What base sequence does it recognize?
它能识别什么碱基序列
Transcription just means making a single stranded RNA copy of a sequence of base pairs in a DNA.
转录的过程就是从一段DNA碱基序列中,复制出一段单链RNA
For example, this transfer RNA has a unit here, at one end of the transfer RNA molecule.
举个例子,这个tRNA的一端,有一个GAG的碱基序列单元
The DNA molecule here has been spread apart, opening up two single stranded DNA's which have complementary base sequences because they were double stranded DNA.
这里DNA分子被分开,形成两条单链DNA,这两条单链碱基序列互补,因为它们是由双链DNA解旋形成的
Well you do that by this Watson-Crick base pairing, so I know if I have I know what messenger RNA to make from that because I have to satisfy these base pairing rules.
依据的是沃森克里克碱基互补配对原则,如果给我一个ACGCGA的序列,就能知道转录出的mRNA会是什么,因为我必须满足碱基配对原则
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