It has to have an origin of replication, which we talked about last time, which is compatible with the cells.

所以质粒必须带有复制起点,我们上次讲过,该起点要与受体细胞匹配

耶鲁公开课 - 生物医学工程探索课程节选

So having a place that the cell knows - where the cell knows how to replicate is important and so plasmids have an origin of replication.

所以得有一个细胞能识别的区域,能使细胞知道,怎样来复制 这很重要,因此质粒上带有一个复制起点

耶鲁公开课 - 生物医学工程探索课程节选

This region here of this particular plasmid is called the ori or origin of replication.

质粒上的这个区域叫,ori,或者叫复制起点

耶鲁公开课 - 生物医学工程探索课程节选

A primer is a short RNA sequence or DNA sequence that gets sort of the process of replication jump started, and that's just because of the biological properties of DNA polymerase that that primer's needed.

引物是一段短的RNA或者DNA序列,引物是整个复制的起点,因为DNA聚合酶的,生物特性,引物在复制过程是必不可少的

耶鲁公开课 - 生物医学工程探索课程节选