The problem is the same, how do I get the gene that I want into only the cells that I want?

同样的问题又来了,怎样准确地,把目的基因只导入特定细胞呢

耶鲁公开课 - 生物医学工程探索课程节选

It was a gene that was causing a cell to be malignant and to divide without control, for example.

例如,一个会使细胞恶化,并不受控制地无限分裂的基因

耶鲁公开课 - 生物医学工程探索课程节选

So this might be a longer lasting, more permanent form of elimination of expression of a particular gene.

所以这似乎是一种持续时间更长,更长久地阻止某个特定基因表达的方法

耶鲁公开课 - 生物医学工程探索课程节选

It's a single gene defect and we know exactly where the genetic defect is in sickle cell.

是一种单基因缺陷,而且我们准确地知道,镰状红细胞贫血的基因缺陷在哪里

耶鲁公开课 - 生物医学工程探索课程节选

You're not fishing through a whole chromosome in order to find what you want, but you're going to a cell that's already enriched in it.

你不是大海捞针地,在一整个染色体中找基因,而是在一个充满了,你感兴趣的基因的细胞里找

耶鲁公开课 - 生物医学工程探索课程节选

What you could do then, if you could deliver these red colored molecules here is you could stop specifically the expression of this particular gene in these particular cells.

接下来你能做的是,如果你能将这些红色的分子送到这里,就能够特异地阻止,特定细胞中特定基因的表达

耶鲁公开课 - 生物医学工程探索课程节选

They do that because they can control the expression of genes.

它们能正确地合成蛋白,是因为它们可以调控基因的表达

耶鲁公开课 - 生物医学工程探索课程节选

We're cloning a gene of interest to us by sort of hijacking that biological mechanism by putting our gene on this plasmid and so our gene gets copied many times, as the plasmid gets copied many times.

我们克隆感兴趣的基因的做法,这个生物机制有点像是绑架,我们把基因绑在质粒身上,随着质粒被多次地复制,我们的基因也一起被多次复制

耶鲁公开课 - 生物医学工程探索课程节选

These are two examples I just gave you of introducing genes that affect the life of a cell, either by making it express a protein that it's not making properly that's important to its life, or by intentionally killing a cell by having it express a fatal gene.

刚才我所举两个例子,都只是影响单一细胞生命过程的,无论是使无法正常表达的重要蛋白,正常表达也好,或是有意地,导入致命的基因杀死细胞也好

耶鲁公开课 - 生物医学工程探索课程节选

This is a much more efficient way to get DNA for a gene that you're interested for a couple of reasons.

用这个办法能更有效率地获取你,感兴趣的基因的DNA,原因如下

耶鲁公开课 - 生物医学工程探索课程节选