The regions that are necessary for describing what the protein is like are called exons, the regions that are not are called introns.

因此我们把编码蛋白质的区域,称为外显子,而把不参与编码蛋白的区域称为内含子

耶鲁公开课 - 生物医学工程探索课程节选

One way that you can look for that gene that you want is by making the cut in your plasmid inside of a gene that encodes for some property like resistance to an antibiotic.

一种找到成功重组质粒的方式,是在质粒控制某种特性的编码区域切割,例如,对抗生素的抗性

耶鲁公开课 - 生物医学工程探索课程节选