If you have DNA fragments, so this is DNA that you've cut up into fragments using restriction enzymes for example.

如果你有DNA片段,例如有一段你用限制酶,切割成片段的DNA

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This gives you a biological mechanism for cutting, using restriction enzymes, and then you denature so that it falls apart, and then you renature so that it comes back together.

这是一种切割DNA的生物机制,用限制性内切酶,改变DNA的性质让它打开,然后让它合起来恢复它的性质

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If I took this same restriction enzyme and tried to cut the chromosomal DNA of a sickle patient, it wouldn't cut at that point because the wrong sequence is there.

而如果我用同样的限制酶,去切割病人的染色体DNA,那该酶是不会起作用的,因为基因序列不对

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If I cut both the plasmid and my DNA of interest with the same restriction enzyme I'm going to end up with the same sticky ends on both molecules.

如果用同一种限制性内切酶,来切割质粒和我感兴趣的DNA,在两个分子上就能得到同样的粘性末端

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