In this case, this particular restriction enzyme cuts symmetrically like this, but not at the same point.

在我们这种情况下,这个限制性内切酶总是这样对称地切割,但两条链切割的位点不一样

耶鲁公开课 - 生物医学工程探索课程节选

The first step is we had to be able to take this circular DNA and cut it to create a site for our new gene to be added.

第一步是切割环形DNA,以创造出一个整合新基因的位点

耶鲁公开课 - 生物医学工程探索课程节选