The best way to label this DNA is by designing probes or labels that hybridize with specific sequences that you're interested in.
最好的标记方法是,设计出能与你感兴趣的,特定基因序列杂交的,分子探针或标签
And he's saying that, you know, "This grasp right now is damaging tissue because it's badly designed, etc."
来到我们课堂的医生会说,“因为仪器设计不当,现在的抓取方法会破坏组织等等。”
What you're much better at doing is saying how do I take the problem I've got and map it into a class of algorithms about which I know and use the efficiencies of those to try and figure out how to make it work.
当你碰到问题的时候,更好的方法是把遇到的问题映射,到已经设计好的算法中去,并用这些算法来提高效率,来保证问题得到解决。
Each one of those electrodes allows us to measure, we plot voltage verses time, ? this signal coming from the tip of that electrode. Ok?
我们通过每个这样的电极都可以设计一种测量方法,我们设计电压比时间,这一信号源于电极的一端,对吧?
Yeah, this is just one particular example of how to do it, but you could - so you have to identify something that's unique about it and then design a method for identifying that unique thing.
是的 这只是一个特定的例子,但你可以--,你必须找到它,独一无二的特性,然后才能设计出识别这种特性的方法
So, that means we have to devise ways of sharing with people that we've never met, that we don't care about--I mean, maybe we care about everybody, but we don't have any particular emotional ties to them-- and they're very different people.
那意味着我们必须设计一些方法,使我们与素未谋面的,并不亲近的人们一同分摊风险,也许我们非常博爱,但未必对所有人都会怀有特殊情感-,分摊者应该是与我们非常不同的人。
up employing something called programming, the act of constructing, telling a computer to do your bidding.
你们会使用一种叫做程序设计的方法,或称之为构建,即让计算机去执行你的命令。
And probably the obvious thing you'll think about is, we'll come up with a clever algorithm. And I want to disabuse you of that notion.
可能你想到的最有效的方法,就是设计一个,聪明点的算法了,这正是我想你们注意到的。
You could imagine strategies where you have multiple resistance genes on a plasmid, resistance to Ampicillin, to Penicillin, to Erythromycin for example, and you design strategies for separating out which cells are carrying the plasmid that you're interested in.
若质粒上有多种抗性基因,你就有很多筛选方法,例如对氨苄青霉素,青霉素,红霉素的抗性,而且你可以设计方法分离出,含有你感兴趣质粒的细胞
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