If I cut both the plasmid and my DNA of interest with the same restriction enzyme I'm going to end up with the same sticky ends on both molecules.

如果用同一种限制性内切酶,来切割质粒和我感兴趣的DNA,在两个分子上就能得到同样的粘性末端

耶鲁公开课 - 生物医学工程探索课程节选

Well one way you could do it is by saying 'if I have this one base pair difference then this sequence is going to be cut by a specific restriction enzyme that recognizes the sequence CTGAGGA'.

一种方法是通过考虑,"如果我有这个不同的碱基对,那该序列必然会,被特定的限制酶识别并切割,这个特定的限制酶能识别,CTGAGGA "

耶鲁公开课 - 生物医学工程探索课程节选