When it cuts it leaves sticky ends or un-base paired single stranded regions on each end of the part its cut and that's just a property of many restriction enzymes; not all, some cut blunt,just right down the middle.

它切割后会留下两个粘性末端,或者未配对的单链部分,这是许多限制性内切酶的特性,但不是全部,有些限制性内切酶是从中间钝切的

耶鲁公开课 - 生物医学工程探索课程节选

Transcription just means making a single stranded RNA copy of a sequence of base pairs in a DNA.

转录的过程就是从一段DNA碱基序列中,复制出一段单链RNA

耶鲁公开课 - 生物医学工程探索课程节选

The DNA molecule here has been spread apart, opening up two single stranded DNA's which have complementary base sequences because they were double stranded DNA.

这里DNA分子被分开,形成两条单链DNA,这两条单链碱基序列互补,因为它们是由双链DNA解旋形成的

耶鲁公开课 - 生物医学工程探索课程节选