Here, the problem is solved by injecting it directly into the cell, shown here, and then that's one issue with gene delivery.

现在,这个问题已经通过,将其直接注入细胞的方法解决了,图上这里,这只解决了基因输送的一个问题

耶鲁公开课 - 生物医学工程探索课程节选

And I responded to at least one email about this issue, someone quite correctly said tuple are immutable, how can I change one?

我在不止一封邮件里回复了这个问题,一些人很明智，知道元组不可变,但是疑问怎么能去改变他们，我的答案是？

麻省理工公开课 - 计算机科学及编程导论课程节选

There's never been a successful third-party political culture take hold in this country without one really big issue to drive it.

美国就从没有过,第三政党真正成功执政的时候,因为缺乏一个真正重大的议题来支持它

耶鲁公开课 - 美国内战与重建课程节选

extending the issue of the two-parent versus one-parent family is, "To what extent are these notions validated " cross-culturally?"

将双亲和单亲家庭的问题扩展了一下，“这些概念究竟在多大程度上,会因跨文化差异而有所变化“

耶鲁公开课 - 心理学导论课程节选

Well, however we settle that issue, notice there's still one other assumption that all these positions still have in common.

不管我们如何解决这个问题，我们都会发现有一个假定,是这些观点都共有的。

耶鲁公开课 - 死亡课程节选

There's one particular issue with overnutrition called the metabolic syndrome that I'd like to talk about and show you a little data on.

代谢综合征是我将讨论并列举数据的,其中一个与营养过剩有关的问题

耶鲁公开课 - 关于食物的心理学、生物学和政治学课程节选

One more issue is, you have another decision when you run up to hit this, hit the penalty other than just left and right.

另一个问题是,在你主罚点球的时候,除了左路右路外还有别的需要考虑吗

耶鲁公开课 - 博弈论课程节选

In many ways for Aristotle as it is for every student of politics the most serious the most difficult issue one confronts is the problem of faction.

从很多方面看来，无论是对亚里士多德,还是每位政治系的同学而言,最令人最感严肃,与严重的问题,是派系的问题。

耶鲁公开课 - 政治哲学导论课程节选

Now, you have to understand that when you buy a bond, if you buy it at issue, you get the first coupon in six months, the second coupon in one year, the third coupon in eighteen months, and the last coupon you get at the maturity date.

你们需要了解,如果在发行时就购入债券,六个月后会得到第一笔票息,一年后第二笔,18个月后第三笔,到期时得到最后一笔票息

耶鲁公开课 - 金融市场课程节选

The issue of connection has social factors and it's one answer that sociologists give for why it's good to go to Yale.

连接性这个问题是有社会因素的,社会学家给出过一个答案,为什么去耶鲁读大学是好事。

耶鲁公开课 - 心理学导论课程节选

Here's another video clip from that same series that speaks to this issue, this one's beautifully done.

这儿是这个系列的另一段视频,也是围绕这个问题的,做的很完美

耶鲁公开课 - 关于食物的心理学、生物学和政治学课程节选

And one personal issue within any of us is what would we do in such situations?

我们所有人都面对的一个个人问题就是,在那种情况下,我们会怎么做

耶鲁公开课 - 心理学导论课程节选

That's a big issue. You can fix one thing, you break something else.

你可以解决一个问题，但你破坏了其它的程序。

麻省理工公开课 - 计算机科学及编程导论课程节选

The second one--the second study illustrates this issue even more clearly.

第二个研究,更加清晰的对该问题进行了阐述

耶鲁公开课 - 心理学导论课程节选

Let me just raise one more issue.

我再来提出一个问题吧

耶鲁公开课 - 博弈论课程节选