It has to have an origin of replication, which we talked about last time, which is compatible with the cells.

所以质粒必须带有复制起点,我们上次讲过,该起点要与受体细胞匹配

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All these components have to be compatible with each other and it has to work according to a plan, which ultimately is informed by this theory.

所有的零部件都要能够相互兼容,并能发挥作用,按照上述理论

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The second issue is that the gene vector, in this case the plasmid, has to be compatible with the cells that you're trying to express the gene in.

接下来的问题是如何选择合适的基因载体,在这里用到的,质粒,必须与要用来表达基因的,受体细胞匹配

耶鲁公开课 - 生物医学工程探索课程节选

You have to be able to get the DNA into the cell, it has to be incorporated into a vector of some sort, and the vector has to be compatible with the species and the cells that you've provided the DNA to.

你必须把整合在,某种特定载体上的DNA导入细胞,载体也必须和,受体物种,及细胞匹配

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