If your gene goes in, you've interrupted the gene for antibiotic resistance and those new organisms aren't going to be resistant to antibiotic anymore.

如果基因进入了质粒,原本对抗生素有抗性的基因就被打断了,那些新细菌就不再对抗生素有抗性

耶鲁公开课 - 生物医学工程探索课程节选

One way that you can look for that gene that you want is by making the cut in your plasmid inside of a gene that encodes for some property like resistance to an antibiotic.

一种找到成功重组质粒的方式,是在质粒控制某种特性的编码区域切割,例如,对抗生素的抗性

耶鲁公开课 - 生物医学工程探索课程节选

I'll tell you, in the south of France near where we live, when there was a lot of resistance in World War II against the Germans, there were some Protestant villages there that were noteworthy for their resistance.

例如在法国南部,当时我们住所的附近,有一些新教徒的村子,这些村民们在二战中奋力抗击德国的入侵,并因为他们的不屈抗争而闻名

耶鲁公开课 - 欧洲文明课程节选

You could imagine strategies where you have multiple resistance genes on a plasmid, resistance to Ampicillin, to Penicillin, to Erythromycin for example, and you design strategies for separating out which cells are carrying the plasmid that you're interested in.

若质粒上有多种抗性基因,你就有很多筛选方法,例如对氨苄青霉素,青霉素,红霉素的抗性,而且你可以设计方法分离出,含有你感兴趣质粒的细胞

耶鲁公开课 - 生物医学工程探索课程节选