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You will find this pattern very useful anytime you're not feeling happy about something and you want to express yourself.
你会发现，当你因为什么事感到低落，你想表达自己的感觉时，这个句型特别有用。
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So if you had some number back in grade school like 123, well, why do we express this as 123?
在小学，像123这样的数，为什么我们要表达成123这样的呢？
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After you part ways, you can turn to your friend and express your frustration that he did not remember you.
你们分开后，你可以向你的朋友表达他不记得你的失落感。
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It's just kind of like the way you express yourself, you know?
就是一种自我表达的方式，你知道吗？
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As a closing remark, you can express your excitement at finally being able to see your loved one after such a long absence.
作为结束的话，你可以表达一下这么久以来即将见到心爱的人的激动心情。
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So, that's very healthy to be social and to, you know, express yourself.
所以，善于社交和表达自我是很健康的。
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You need to find a way to express yourself.
你得找到一种表达自己的方式。
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Remember you can say "I'm sorry" when you wish to express your regret, your sympathy or ambiguous regret.
记住，当你想表达歉意、同情或是含糊的道歉时，你都可以用“I'm sorry”。
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So if it's not a cell that's going through division you can't use a retrovirus to express the protein.
如果细胞不在分裂阶段,你就不能用逆转录病毒来表达某个蛋白
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- And so you can begin to express -- -- even though the language is a bit arcane -- your ideas in the same way that you might actually speak them.
所以你可以开始表达-,即使那个语言有点晦涩-,你的想法可以用同样的方式说出来。
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And instead of feeling uncomfortable, you can express your discomfort by saying,
要是这个玩笑让你感觉不自在，你可以这样表达你的不满：
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the easier it comes when you are standing in front of people and you have to express yourself,
你会发现，当你站在一群人面前，得表达自己观点的时候，一切会变得更容易，
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Use this pattern whenever you wish to express your intention to do or not to do something.
只要你想表达是否打算做某事，都能使用这个句型。
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Of course the most common use of the "I want to" pattern is when you express your desire for something.
当然，“I want to”句型最常见的用法是用来表达你对某事的愿望。
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When it does happen, when the idea comes and you wrestle to place the words that express exactly what it is that you want to say, what it is that the meaning you're wrestling for.
当其真的发生时,你绞尽脑汁,希望写出,能准确表达它的文字，你苦思冥想，究其含义。
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And since the city, you remember, is simply the soul at large, the three classes of the city merely express the three parts of the soul.
而因为城市，简言之，即是灵魂的放大版，城市的三个阶级,因此即代表着灵魂的三个部份。
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Yeah, but I think it's also an important skill. You need to be able to express what you know
难，但是我也觉得这是一种重要的技能。你必须能够把你知道的表达出来，
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it's very difficult when one is so articulate and is able to express exactly what you want to say,
当一个人已经熟谙一门语言，能够用这门语言清楚明白地表达意思，
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You use this pattern whenever you want to express that you have experienced something in the past previously.
每当你想表达你之前已经经历过某事的时候，你就可以用这个句型。
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And it's a useful pattern when you want to express that your feelings have been hurt or that you're not liking something,
当你想表达你在情感上受到了伤害或者不喜欢某件事的时候，这是个很有用的句型。
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It can have a very sarcastic undertone which could be good, depending on what you need to express.
它可以表达一种带着挖苦意味的好意，这取决于你表达的需要。
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You can use this to express possession or a lack of possession of anything. Tangible or intangible.
你可以用它来表示你有或没有什么东西。有形与无形的事物都可以通用。
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Use the "I'm frustrated" pattern to express yourself when you feel stifled,
当你觉得很压抑的时候，你可以使用“I'm frustrated”句型。
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So with eight bits can you express 256 possible characters?
用八个比特你就可以表达256个可能的字符？
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So it's going to express this gene, but you've put a foreign gene in the place of the natural gene, and so the foreign gene gets expressed instead.
所以它就会表达这段基因,但是你已经用一段,异源基因替代了原有基因,因此这段外来基因就被表达了
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The second issue is that the gene vector, in this case the plasmid, has to be compatible with the cells that you're trying to express the gene in.
接下来的问题是如何选择合适的基因载体,在这里用到的,质粒,必须与要用来表达基因的,受体细胞匹配
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But mathematically, we've mentioned this before, log N or really to be precise, log base 2 of N, is the way you express this mathematically.
但从数学上说，之前我们已经提到过了，准确地说是log，N，以2为底N的对数，这就是它在数学上的表示。
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There is, in the first set of dialogues, a distinct hierarchy of characters, you might say, who we see later on express those distinctive features of the soul and the city.
在第一段对话中，可以明显地看出角色间的尊卑地位，你也可以说这些人,所表达出的独特性格,正是灵魂与城邦所具备。
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