Sometimes a blunt cut is useful if you want to sort of grow a sticky end of your choice on it.

这时候钝切口很有用,尤其在你想获得选定的粘性末端的时候

耶鲁公开课 - 生物医学工程探索课程节选

And not just a camera but there are also tools on the end of these things so you can cut and you can do manipulations through this instrument.

除了摄像头外,在关节镜的末端还有其他的器械,你可以体外操纵这些器械进行切割等操作

耶鲁公开课 - 生物医学工程探索课程节选

If everyone else is cutting down I don't have too, and if everyone else does cut down I don't have to, I end up using hot water and driving a big car and so on.

如果大家都想减排,我没必要跟风,如果别人真的减排了又与我何干,我照样用着热水,开着大排量汽车

耶鲁公开课 - 博弈论课程节选

When it cuts it leaves sticky ends or un-base paired single stranded regions on each end of the part its cut and that's just a property of many restriction enzymes; not all, some cut blunt,just right down the middle.

它切割后会留下两个粘性末端,或者未配对的单链部分,这是许多限制性内切酶的特性,但不是全部,有些限制性内切酶是从中间钝切的

耶鲁公开课 - 生物医学工程探索课程节选

If I cut both the plasmid and my DNA of interest with the same restriction enzyme I'm going to end up with the same sticky ends on both molecules.

如果用同一种限制性内切酶,来切割质粒和我感兴趣的DNA,在两个分子上就能得到同样的粘性末端

耶鲁公开课 - 生物医学工程探索课程节选