-
Now in this case the host - you want the host not only to replicate all the DNA, you want it also to express the gene.
而这种情况下宿主--,你不只希望宿主复制基因,同时也想使之表达基因
-
If you repeatedly go through this cycle of denaturing, synthesizing, denaturing, synthesizing you'll get many, many copies of DNA.
如果你不断重复这个变性-复制,变性-复制的循环过程,你就会得到很多很多的DNA复制产物
-
That's one of the properties of mitosis, that the DNA gets completely duplicated during the S phase, during DNA synthesis phase.
有丝分裂的一个特征,就是DNA在S期内完全复制,S期就是DNA合成期
-
You're not just trying to clone the DNA, you're trying to also clone or make many copies of the protein.
你不仅想要复制DNA,更想要克隆或是制造,许许多多的蛋白质产物
-
You have a fragment of some kind of DNA that you would like to clone or make many copies of.
你有了某种DNA的片段,想要克隆,复制它
-
Transcription just means making a single stranded RNA copy of a sequence of base pairs in a DNA.
转录的过程就是从一段DNA碱基序列中,复制出一段单链RNA
-
They enter the cell after recognizing, they fall apart inside the cell, they reproduce their DNA, or their genetic material using host mechanisms.
在识别细胞后侵入之,它们在细胞内解体,复制自身的DNA,利用宿主的遗传来机制复制自己的DNA
-
You first separated your DNA, let the primers bind, then turn on the enzyme, and it makes copies of each one of these.
首先解旋DNA双链,使引物结合,接着激活聚合酶,最终得到各链的复制产物
-
That's synthesis and that has to happen in order for cells to replicate and cell replication is happening in your body all the time.
这个DNA合成过程是,细胞复制中不可或缺的部分,细胞复制时刻不停的在你身体里进行着
-
Before, we talked about how do you know what messenger RNA to make, how do you know what RNA to copy from a DNA template?
在之前的课上,我们说到如何判断mRNA的具体序列,如何判断RNA从DNA模板复制了什么
-
that means that the cells have to be able to replicate the DNA on the plasmids, in order to make many more copies of it.
就是说这些细胞要能够,复制质粒上的DNA,以得到更多的拷贝
-
A new single stranded DNA is formed on each one of these open single strands.
对两条打开的单链进行复制,会生成两条新的DNA单链
-
The result of this process if this replication went down the whole length of the DNA would be to form two identical, double stranded DNA molecules.
如果复制过程在整条链上持续进行的话,将生成两条完全一样的,双链DNA分子
-
What's shown here is what's called a replication fork in DNA that's undergoing synthesis.
这里展示的是在DNA合成过程中,形成的DNA复制叉
-
It's totally synthetic, there's no micro-organism involved in making the DNA for you, You can do it very rapidly in a laboratory, and fairly inexpensively now.
完全是复制的过程,没有任何微生物参与制造DNA,你可以在实验室里迅速地进行,费用现在也相当便宜了
-
So DNA is replicated using one strand of the DNA as a template.
所以DNA复制是以DNA单链为模板的
-
DNA cloning, or any kind of cloning just means 'making copies of'.
NA克隆,或者任何一种克隆,本义就是"复制"
-
We want not only for the cell to be able to synthesize the DNA, you want it to be able to express the protein off the DNA as well.
我们不仅希望细胞能复制DNA,还希望它能表达目标DNA所编码的蛋白
-
The polymerase starts a process of replication of your DNA sequences.
聚合酶使DNA序列开始复制
-
Remember we talked about how DNA replicates itself and that there are enzymes, DNA polymerase that bind to the double stranded DNA, separate it, denature it locally, and then start the process of replication.
请记住DNA复制必须有酶的参与,DNA聚合酶结合在双链DNA上,打开双链并原位变性,然后才开始复制
-
So the process of cloning DNA is taking a few strands of DNA of a gene that you're interested in and making many copies of them, that's cloning,you like to make identical copies.
所以DNA克隆的过程,就是用你所感兴趣的,基因的一些DNA链,来得到更多它们的拷贝,这就是克隆,复制出相同的拷贝
-
A primer is a short RNA sequence or DNA sequence that gets sort of the process of replication jump started, and that's just because of the biological properties of DNA polymerase that that primer's needed.
引物是一段短的RNA或者DNA序列,引物是整个复制的起点,因为DNA聚合酶的,生物特性,引物在复制过程是必不可少的
-
Let's assume that we have this plasmid cloning vector and we have some pieces of DNA that we would like to put into a plasmid that we would like to make copies of.
假设我们已经有了克隆质粒载体,也有了我们想要将之,整合到质粒上的某些DNA片段,同时也是我们所要复制的DNA片段
-
That DNA gets replicated to make many more copies of the viral DNA using host mechanisms, that is, often using the DNA polymerase which is naturally present in the host cell for its own replication.
病毒DNA进行复制,通常是利用在宿主细胞自身的有关物质,利用宿主细胞中天然存在的,DNA聚合酶来大量复制病毒DNA
-
Because HIV is an RNA virus and if it enters your cell the only way it can replicate, it can put its DNA into your cells, is by first making DNA out of its RNA genome.
因为HIV是RNA病毒,如果它进入你的细胞,它复制自己的唯一方式,它能把自己的DNA转入你的细胞,方法是先用自己的,RNA基因组制造出DNA
-
Synthesis always occurs in one direction and that makes sense to you now because you know there's a directionality and the chemistry is different going one way than the other and this DNA polymerase only works on the chemistry going in one direction.
NA复制过程是单向的,现在你们该明白原因了,因为DNA单链有方向性,DNA在两个方向上化学性质不同,DNA聚合酶,只在一个方向上起作用
-
Certain segments of DNA are being converted into RNA, and whereas in replication, you have to copy the whole genome, the whole - all of the chromosomes, all of the DNA contained in the chromosomes of the cell in order to completely replicate it; transcription only works on particular sequences of DNA.
特定的DNA序列被转录为RNA,在复制的过程中,整条染色体都被复制,包括其中的全部DNA序列,都被分毫不差地复制下来,以保证复制的完整性,而转录过程只发生在特定的DNA序列上
应用推荐