You then denature again, each one of these strands gets separated, primers bind, turn on the polymerase, a new strand is made.

然后你可以再次使产物链变性,使各链解旋,再结合引物,激活聚合酶,再生成新链

耶鲁公开课 - 生物医学工程探索课程节选

You denature them, you can watch them denature because the egg white turns from clear to white and it doesn't go back.

它们变性了,这种变性是可以观察到的,蛋清由透明变为白色,再也变不回去

耶鲁公开课 - 生物医学工程探索课程节选

Now, you all know that we can make irreversible changes in proteins, you can denature them completely, that's what happens when you cook an egg for example.

现在你们都懂蛋白能够发生不可逆的改变,能够完全变性,比如在你煮鸡蛋的时候就是这样

耶鲁公开课 - 生物医学工程探索课程节选

If I took two double stranded DNA molecules and I exposed them to certain conditions that caused them to denature, that means its native structure falls apart.

假如我把两条DNA双链,放在特定的条件下,使它们变性,变性表示使它们的原始结构分开

耶鲁公开课 - 生物医学工程探索课程节选

This gives you a biological mechanism for cutting, using restriction enzymes, and then you denature so that it falls apart, and then you renature so that it comes back together.

这是一种切割DNA的生物机制,用限制性内切酶,改变DNA的性质让它打开,然后让它合起来恢复它的性质

耶鲁公开课 - 生物医学工程探索课程节选

Remember we talked about how DNA replicates itself and that there are enzymes, DNA polymerase that bind to the double stranded DNA, separate it, denature it locally, and then start the process of replication.

请记住DNA复制必须有酶的参与,DNA聚合酶结合在双链DNA上,打开双链并原位变性,然后才开始复制

耶鲁公开课 - 生物医学工程探索课程节选