Some time around this period of time,in early Christianity, somebody got the big idea, "Hey,let's cut up the scroll into pages, and sew the pages together. And then,put it all in a book.
大概在这段时期,在早期的基督教里,有人想出了一个好主意,即把书卷断成张页,并用线一张张连起来使之成书。
You just have to live, you can't cut up.
你不得不生存,你不能就此停止。
If you have DNA fragments, so this is DNA that you've cut up into fragments using restriction enzymes for example.
如果你有DNA片段,例如有一段你用限制酶,切割成片段的DNA
If everyone else is cutting down I don't have too, and if everyone else does cut down I don't have to, I end up using hot water and driving a big car and so on.
如果大家都想减排,我没必要跟风,如果别人真的减排了又与我何干,我照样用着热水,开着大排量汽车
Well the first step would be to cut open the plasmid with a particular restriction enzyme, and then what if I take that same restriction enzyme and I cut up the DNA that I'm interested in.
第一步是用某种限制性内切酶把质粒切开,然后用同一种限制性内切酶,切出我想要的DNA
I could cut that up into fragments, and I could search in these fragments to try to find the one that has the insulin gene on it.
我把那部分切成碎片,然后从中找到有胰岛素基因的那一段
If I cut both the plasmid and my DNA of interest with the same restriction enzyme I'm going to end up with the same sticky ends on both molecules.
如果用同一种限制性内切酶,来切割质粒和我感兴趣的DNA,在两个分子上就能得到同样的粘性末端
In a normal cell DNA that DNA in the normal cell - this DNA gets cut by the restriction enzyme, so the sickle gene ends up - so that hemoglobin gene ends up in two pieces.
一个正常细胞的DNA,正常细胞里的DNA,会被限制酶所剪切,因此镰状血红蛋白基因--,不对,是正常血红蛋白基因被切成两段
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