That they agree to some kind of base that's why I said that things maybe murkier in the end than we want.

然后他们就打成某种协议,这也就是为什么我说最终事情会变得更加复杂,虽然我们并不希望这样。

普林斯顿公开课 - 国际座谈会课程节选

When it cuts it leaves sticky ends or un-base paired single stranded regions on each end of the part its cut and that's just a property of many restriction enzymes; not all, some cut blunt,just right down the middle.

它切割后会留下两个粘性末端,或者未配对的单链部分,这是许多限制性内切酶的特性,但不是全部,有些限制性内切酶是从中间钝切的

耶鲁公开课 - 生物医学工程探索课程节选