If everyone else is cutting down I don't have too, and if everyone else does cut down I don't have to, I end up using hot water and driving a big car and so on.
如果大家都想减排,我没必要跟风,如果别人真的减排了又与我何干,我照样用着热水,开着大排量汽车
Now what I'd like to do is something that you'll probably cut out because of copyright issues but it's a kind of fun warm-up anyway, so we're going to go ahead and do this and then we'll actually start.
首先我想做个小游戏,不过这个片段可能由于版权问题而被切掉,但是这个热场游戏很有趣,那么,我们马上开始,游戏之后再正式上课
If you cut and you open up a segment of DNA then you've left these sticky ends, for example and these sticky ends are capable of recognizing each other by the process of hybridization.
如果你进行切割打开一个DNA片段,就会留下,比如说,这些粘性末端,这些粘性末端能在杂交的过程中互相识别
Well the first step would be to cut open the plasmid with a particular restriction enzyme, and then what if I take that same restriction enzyme and I cut up the DNA that I'm interested in.
第一步是用某种限制性内切酶把质粒切开,然后用同一种限制性内切酶,切出我想要的DNA
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