Like we talked about with insulin; make large quantities of this recombinant viral protein, and use that as a vaccine.

就像我们讲过的胰岛素,生成大量重组的病毒蛋白,并将其作为疫苗使用

耶鲁公开课 - 生物医学工程探索课程节选

If it was directly transcribed there'd be regions that are important for making insulin and regions that are not.

如果直接转录的话,那被转录的序列中就会既包括了,和生成胰岛素有关的重要区域,也包括了与之无关的区域

耶鲁公开课 - 生物医学工程探索课程节选

If I'm going to make insulin from this I have to know that I've got the introns spliced out correctly.

如果我要用这些来生成胰岛素,我就得知道是不是把内含子都准确剔除了

耶鲁公开课 - 生物医学工程探索课程节选

There are more steps that have to happen correctly in order for that insulin to become active.

还有几个步骤,必须正确完成这些步骤后,才能生成有活性的胰岛素

耶鲁公开课 - 生物医学工程探索课程节选

Only cells of your pancreas make insulin, for example.

举例来说,只有胰腺细胞生成胰岛素

耶鲁公开课 - 生物医学工程探索课程节选

If I want to clone a gene from a human, if I want to clone the gene for human isulin, mean make many copies of the gene that's responsible for making insulin, I need to know whether there are introns there or not.

如果我想要从一个人类个体克隆基因,如果我想要克隆人类胰岛素基因,亦即我要把负责生成胰岛素的基因,复制许多个副本,我就得要知道哪些是内含子,哪些不是

耶鲁公开课 - 生物医学工程探索课程节选

Those regions that are not are spliced out during RNA processing to form the mRNA transcript that's used to make the protein.

而这些与生成胰岛素无关的区域,在RNA剪接过程中就被剔除,从而生成转录蛋白质的mRNA

耶鲁公开课 - 生物医学工程探索课程节选