Because of the lack of oxygen getting to the cells, the cells are no longer able to carry on their various metabolic processes.

由于细胞中缺少氧气,所以细胞不能完成,它们的各种新陈代谢活动

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Now when we put insulin in this place behind the right promoter, the cell thinks that it still has the lacZ gene present which it needs for its metabolism.

现在我们把胰岛素基因,放在正确的启动子后面,宿主细胞就会认为,它仍然有新陈代谢所需的lacZ基因

耶鲁公开课 - 生物医学工程探索课程节选