The sources are: one,the plasmid vector that we've picked, and the second is these genes that for some reason we're interested in.

第一个是我们选择的质粒载体,第二个是我们感兴趣的基因

耶鲁公开课 - 生物医学工程探索课程节选

And in terms of thinking about it for organic chemistry or if you're interested in thinking about the mechanism maybe by which it is toxic, a first step would be to draw its Lewis structure.

而为了在有机化学中思考它,或者如果你对它,有毒的机理感兴趣的话，第一步要做的应该就是画出它的路易斯结构。

麻省理工公开课 - 化学原理课程节选

The first step is to take the DNA fragments that we're interested in and put them into this vector by basically cutting open the double stranded DNA and inserting the gene that we like in the region where we've cut.

第一步是把我们感兴趣的DNA片段,整合到这个载体里,基本的方法是切开质粒这个双链DNA,把我们想要的基因插入切开的区域

耶鲁公开课 - 生物医学工程探索课程节选