So in this case, one of the standard ways to do this, is to use what's called a linked list.
标准的处理方法是使用链表,我接下来也准备这么做。
You can do that by gently adding heat and making it basic, but you make the two DNA strands separate.
你可以通过逐渐加温并使之碱化的方法,使双链DNA解旋
The first step is to take the DNA fragments that we're interested in and put them into this vector by basically cutting open the double stranded DNA and inserting the gene that we like in the region where we've cut.
第一步是把我们感兴趣的DNA片段,整合到这个载体里,基本的方法是切开质粒这个双链DNA,把我们想要的基因插入切开的区域
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