You can do that by gently adding heat and making it basic, but you make the two DNA strands separate.

你可以通过逐渐加温并使之碱化的方法,使双链DNA解旋

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You first separated your DNA, let the primers bind, then turn on the enzyme, and it makes copies of each one of these.

首先解旋DNA双链,使引物结合,接着激活聚合酶,最终得到各链的复制产物

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The DNA molecule here has been spread apart, opening up two single stranded DNA's which have complementary base sequences because they were double stranded DNA.

这里DNA分子被分开,形成两条单链DNA,这两条单链碱基序列互补,因为它们是由双链DNA解旋形成的

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When they separate, the primers that you've added automatically bind through the process of hybridization, and then you turn up the temperature to the optimum for Taq polymerase and DNA synthesis starts.

当双链解旋后,加入的引物就会在杂交的步骤中自动,和解旋后的DNA结合,然后你再将温度升高至,Taq酶的最适温度,DNA就开始合成了

耶鲁公开课 - 生物医学工程探索课程节选

It operates in a similar fashion to DNA polymerase in that it denatures locally or opens up the double stranded DNA, but it's different in that it creates a new polymer from the DNA template in the language of RNA, using RNA nucleotides and not DNA nucleotides.

它的作用机制类似于DNA聚合酶,在局部使DNA双链变性,解旋,但不同的是,它合成了一个基于DNA模板的,使用RNA编码语言的新聚合物,即使用核糖核苷而非脱氧核糖核苷

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