Take a plasmid, cut it open, insert a gene that we want into the plasmid, and then put that plasmid in a host cell, and let the host replicate it.

取一个质粒,把它切开,把目标基因放进去,然后把这个质粒植入到宿主细胞,让宿主来进行复制

耶鲁公开课 - 生物医学工程探索课程节选

The first step is to take the DNA fragments that we're interested in and put them into this vector by basically cutting open the double stranded DNA and inserting the gene that we like in the region where we've cut.

第一步是把我们感兴趣的DNA片段,整合到这个载体里,基本的方法是切开质粒这个双链DNA,把我们想要的基因插入切开的区域

耶鲁公开课 - 生物医学工程探索课程节选