Still,Andy and Jeff often spoke about the trouble of cutting open envelopes and taking the money out.

VOA: special.2010.04.24

The first step is to take the DNA fragments that we're interested in and put them into this vector by basically cutting open the double stranded DNA and inserting the gene that we like in the region where we've cut.

第一步是把我们感兴趣的DNA片段,整合到这个载体里,基本的方法是切开质粒这个双链DNA,把我们想要的基因插入切开的区域

耶鲁公开课 - 生物医学工程探索课程节选