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She was very independent and not afraid to express her thoughts and opinions.
VOA: special.2009.01.11
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The purpose of new form is not to express new content, but to change an old form which has lost its aesthetic quality lost its power to take the film away from our eyes].
新形式的目的不是要表达新的内容，而是要更换已经失去美学价值的旧形式,不能调整我们看世界的旧眼光了。
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In the next example you can use "I'm sorry" not to express regret for your personal fault.
下一个例句中，“I'm sorry”的使用并不是用来表达对个人过失的抱歉。
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The family members were not afraid to express their liberal opinions.
VOA: special.2009.09.20
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So if it's not a cell that's going through division you can't use a retrovirus to express the protein.
如果细胞不在分裂阶段,你就不能用逆转录病毒来表达某个蛋白
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"I was in college when I came to United States and it was interesting that in America they celebrate people in love and express love to each other, because in China people are very reserved and they do not show appreciation openly.
VOA: special.2010.02.08
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The account of the conquest in Joshua 2 through 12, is concerned to express the basic idea that Israel's victories would not have been possible without Yahweh, without his wondrous help.
在《约书亚书》第2到12章中记叙的征服，想要表达一种基本的观点,以色列不可能获胜，如果没有耶和华，没有他神奇的帮助。
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There is not a subject you can raise that he does not respond and know what he wants to express.
VOA: standard.2009.10.14
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You will find this pattern very useful anytime you're not feeling happy about something and you want to express yourself.
你会发现，当你因为什么事感到低落，你想表达自己的感觉时，这个句型特别有用。
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"While this bill is not related to the ongoing post-election events in Iran, we would be remiss to not express our solidarity with the protesters persuing electoral freedom in Tehran, consistent with our democratic values,"
VOA: standard.2009.06.23
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After you part ways, you can turn to your friend and express your frustration that he did not remember you.
你们分开后，你可以向你的朋友表达他不记得你的失落感。
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Fast forward mid-semester when we talk about cryptography and scrambling information, we need the ability to express ourselves in words, not just numbers, and we have that already.
回想一下期中的时候，我们讨论过密码学,和一些不规则信息,我们需要话语来表达自己，而不是数字，其实我们已经有了。
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It's a very easy, very direct, not at all impolite way to express your desires in any situation.
这是个非常简单而直接的句型，用这样的句型在各种情形下表达你的愿望也不会显得不礼貌。
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Because it will allow you to express strong feelings about not liking something or not wanting something.
因为它能帮助你表达一种厌恶或不想做某事的强烈情绪。
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Use this pattern whenever you wish to express your intention to do or not to do something.
只要你想表达是否打算做某事，都能使用这个句型。
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This is great when you want to express regret for something that you perhaps did not do.
当你想表达对一些过去没做成的事情感到后悔时，你可以使用这个句型。
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to express not completely understanding anything, really in any situation.
在任何情况下表达对任何事的不理解。
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As I mentioned, you can also use "I wish" when you express something that you do not want to have happen.
就像我之前提到的，当你想说明你不希望发生的事情时，你也可以用“I wish”。
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You can also use the "I didn't" pattern to express that the result was not the same as your intentions.
你也可以用“I didn't”句型来表达事情的结果并不是你的本意。
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Now in this case the host - you want the host not only to replicate all the DNA, you want it also to express the gene.
而这种情况下宿主--,你不只希望宿主复制基因,同时也想使之表达基因
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And it's a useful pattern when you want to express that your feelings have been hurt or that you're not liking something,
当你想表达你在情感上受到了伤害或者不喜欢某件事的时候，这是个很有用的句型。
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We want not only for the cell to be able to synthesize the DNA, you want it to be able to express the protein off the DNA as well.
我们不仅希望细胞能复制DNA,还希望它能表达目标DNA所编码的蛋白
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These are two examples I just gave you of introducing genes that affect the life of a cell, either by making it express a protein that it's not making properly that's important to its life, or by intentionally killing a cell by having it express a fatal gene.
刚才我所举两个例子,都只是影响单一细胞生命过程的,无论是使无法正常表达的重要蛋白,正常表达也好,或是有意地,导入致命的基因杀死细胞也好
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