I apply ligase, and I've got the plasmid that I had before but now with my gene, colored green here,inserted.

我用连接酶,就能在质粒中把基因,这里绿色的部分,插进去

耶鲁公开课 - 生物医学工程探索课程节选

Not every plasmid in your test tube is going to have the right gene inserted in the right way.

不是试管里的每个质粒,都会与正确的DNA片段重组

耶鲁公开课 - 生物医学工程探索课程节选