Retroviruses, for example, can only insert - can only transduce and express their DNA in cells that are dividing.
就以逆转录病毒为例,它们只能在分裂阶段的细胞里,转导并表达自己的基因
If I want the values two, six and eight in there, I would do an insert of two into that set, an insert of six into that set, and an insert of eight into the set.
如果我想让值2,6,8在那个位置,我会把2插入到那个集合中,把6插入到那个集合中,把8插入到那个集合中。
This is an unfortunate thing you see throughout education frankly, certainly at the secondary and primary school level where you just insert technology into the classroom and expect to solve problems, that is not in life always the case.
遗憾的是,中小学教育中,就是讲知识然后运用所学的知识解决问题,但是实际生活中不总是这样。
The first step in the process is to take our plasmid which we've selected, and to insert the gene that we want into it.
这个过程的第一步,是取得我们选择的质粒,把我们需要的基因插入到里面
Take a plasmid, cut it open, insert a gene that we want into the plasmid, and then put that plasmid in a host cell, and let the host replicate it.
取一个质粒,把它切开,把目标基因放进去,然后把这个质粒植入到宿主细胞,让宿主来进行复制
This is where - this is the region of the plasmid where we're going to insert the DNA that we're interested in.
这就是我们将在质粒上,插入我们感兴趣的DNA的位置
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