The best way to label this DNA is by designing probes or labels that hybridize with specific sequences that you're interested in.
最好的标记方法是,设计出能与你感兴趣的,特定基因序列杂交的,分子探针或标签
Now if I put them in contact with one another, the plasmid that's been opened and fragments of the DNA - special fragments that I've produced with the same restriction enzyme, they'll have the same sticky ends, they will naturally hybridize with one another.
如果我让它们互相接触,已经切开的质粒和DNA片段--,这DNA片段是我已用同种限制性内切酶,处理过的特别片段,这两者会有相同的粘性末端,它们会自然地相互杂交
So, if we hybridize just these three orbitals, what we're going to end up with is our s p 2 hybrid orbitals.
我们会看到现在有3个未配对的电子,可以成键。
And if we hybridize these orbitals in carbon, what we end up with is having two hybrid orbitals, and then we're going to be left with two of our p orbitals that are each going to have an electron associated in them.
如果我们杂化碳原子里这些轨道,我们能得到两个杂化轨道,另外剩下两个p轨道,每个里面有一个电子。
So if we don't have to hybridize one of the p orbitals, we can actually end up with a lower energy situation, because now these s p 2 orbitals are 1/3 s character, and only 2/3 p character, instead of 3/4.
我们的杂化轨道就有更多的p轨道成分,所以它们的能量更高,如果我们不杂化这个p轨道,我们可以得到一个能量更低的情况。
应用推荐