Now in this case the host - you want the host not only to replicate all the DNA, you want it also to express the gene.

而这种情况下宿主--,你不只希望宿主复制基因,同时也想使之表达基因

耶鲁公开课 - 生物医学工程探索课程节选

So it's going to express this gene, but you've put a foreign gene in the place of the natural gene, and so the foreign gene gets expressed instead.

所以它就会表达这段基因,但是你已经用一段,异源基因替代了原有基因,因此这段外来基因就被表达了

耶鲁公开课 - 生物医学工程探索课程节选

The second issue is that the gene vector, in this case the plasmid, has to be compatible with the cells that you're trying to express the gene in.

接下来的问题是如何选择合适的基因载体,在这里用到的,质粒,必须与要用来表达基因的,受体细胞匹配

耶鲁公开课 - 生物医学工程探索课程节选

These are two examples I just gave you of introducing genes that affect the life of a cell, either by making it express a protein that it's not making properly that's important to its life, or by intentionally killing a cell by having it express a fatal gene.

刚才我所举两个例子,都只是影响单一细胞生命过程的,无论是使无法正常表达的重要蛋白,正常表达也好,或是有意地,导入致命的基因杀死细胞也好

耶鲁公开课 - 生物医学工程探索课程节选

There are molecules in cells that give the DNA the signal that it's time to transcribe and express a gene, those are called transcription factors, we'll talk about them a bit later.

在细胞中有些分子能够,给DNA发出信号告诉它,是时候转录和表达某基因了,这些分子叫做转录因子,晚些时候我们会讲它们

耶鲁公开课 - 生物医学工程探索课程节选