Get a gene, put it in a plasmid, get that plasmid expressed in a host, could even be a bacterial host,right?

把一个基因导入一个质粒,让质粒在宿主中表达,这个宿主甚至可以是细菌,对吧

耶鲁公开课 - 生物医学工程探索课程节选

For example, a gene that makes a cell cancerous, I'd like to stop it from being expressed.

举例来说,如果某段基因导致细胞癌变,我就希望阻止它表达

耶鲁公开课 - 生物医学工程探索课程节选

You would like to get the gene that you're interested in expressed in some population of cells within the body but not in other cells.

我们希望目的基因,在一些细胞中表达,在另一些中不表达

耶鲁公开课 - 生物医学工程探索课程节选

So it's going to express this gene, but you've put a foreign gene in the place of the natural gene, and so the foreign gene gets expressed instead.

所以它就会表达这段基因,但是你已经用一段,异源基因替代了原有基因,因此这段外来基因就被表达了

耶鲁公开课 - 生物医学工程探索课程节选

When a gene gets expressed, that means its protein is produced.

一段基因得到表达,就意味着合成它所编码的蛋白

耶鲁公开课 - 生物医学工程探索课程节选

Taking foreign genes, genes that aren't naturally expressed or might not even exist inside a cell and putting them there and putting them there in a way where they work, and by work meaning the gene gets expressed or translated into a protein.

将异源基因,即那些在细胞中原本不表达或者,根本不存在的基因转入细胞并让它们工作,工作就是指让基因持续表达并合成蛋白质

耶鲁公开课 - 生物医学工程探索课程节选

These are ways to stop a gene from being expressed, and there turns out there's lot of applications in that, lots of diseases result from the unwanted expression of certain kinds of genes and cancer is probably the best example of that, but there are many.

有些方法能够终止基因的表达,而这些方法也经证实有广泛的应用,有很多疾病是源于基因的异常表达,而癌症大概是这种情况最好的例子,但还有很多其他的

耶鲁公开课 - 生物医学工程探索课程节选

This is introducing a gene, it's a natural gene but it's not - it's a natural gene that's found in humans and is actually expressed all over your body in different levels, but you're concentrating it or over expressing it in one particular region of tissue in order to have a particular effect.

通过导入一个基因,这是一个天然基因但--,是一种于人体内发现的天然基因,在全身细胞中都有不同程度表达,但需要集中在组织的某一特定区域,表达或过表达,才能达到特定疗效

耶鲁公开课 - 生物医学工程探索课程节选

You've heard about RNA interference, for example, and that is the process of stopping this, to stop a gene from being expressed.

比如说,你们听说过的RNA干扰,就是一种阻止,基因表达的手段

耶鲁公开课 - 生物医学工程探索课程节选

It requires RNA processing to happen smoothly, so if you can interfere with RNA processing you can stop a gene from being expressed.

转录需要RNA的顺利加工,因此如果你能干扰RNA加工过程,你就能阻止一段基因的表达

耶鲁公开课 - 生物医学工程探索课程节选

If you can interfere with any stage in RNA processing you can stop a gene from being expressed and this is a very hot topic in molecular biology now and human therapeutics.

如果你能干扰RNA加工的任何一个阶段,你就能阻止一段基因的表达,这也是当今分子生物学,和新型疗法中非常热门的话题

耶鲁公开课 - 生物医学工程探索课程节选